Soltani Saber, Mozhgani Sayed-Hamidreza, Roohinezhad Roozbeh, Emami Setareh Hedayati, Emami Mah Hedayati, Solooki Setayesh, Fattah Hesari Mina, Doroozeh Niloofar, Norouzi Mehdi
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Microbiology and Virology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
AIDS Res Hum Retroviruses. 2025 Mar;41(3):113-119. doi: 10.1089/AID.2024.0010. Epub 2024 Aug 8.
Human T cell lymphotropic virus type 1 (HTLV-1) is associated with adult T cell leukemia/lymphoma (ATLL), a fetal malignant infection. Recently, HTLV-1 new asymptomatic carriers (ACs) have frequently been reported among blood donors. Reaching the profound concept of HTLV-1-associated molecular pathogenesis could result in finding novel therapeutic strategies. The current study aimed to determine leukemia-related signaling regulation in ATLL. Thirty participants were evaluated in 3 groups, including 10 ATLL patients, 10 ACs, and 10 normal controls. Blood samples were isolated without any chemotherapy history from ATLL patients. Also, blood samples were recovered from ACs and normal individuals. White blood cells isolation was done on the collected blood samples. After this, RNA was extracted from the prepared samples and used for the cDNA synthesis. and HTLV-1 basic leucine zipper factor as viral genes and cellular genes, including , , , , , , , and , were investigated using real-time PCR. The mean age of ATLL patients was 53.2 ± 7.32 years, and 9 (90%) were male. The and expression levels were significantly associated with ATLL patients compared with the internal control. However, the significant differences in expression of other genes in the remaining groups were not seen. Discovering viral and cellular signaling pathways that regulate HTLV-1 transformation is essential. A novel therapeutic strategy for ATLL-regulating cellular signaling pathways could be considered. Therefore, clinical trials using activators and inhibitors of related cellular signaling pathways for cell therapy of ATLL are recommended. It is recommended that more investigation be conducted on and to target these genes and reveal the molecular pathogenesis of ATLL.
人类嗜T淋巴细胞病毒1型（HTLV-1）与成人T细胞白血病/淋巴瘤（ATLL）相关，这是一种致命的恶性感染。最近，在献血者中频繁报道了HTLV-1新的无症状携带者（ACs）。深入了解HTLV-1相关的分子发病机制可能会带来新的治疗策略。当前的研究旨在确定ATLL中与白血病相关的信号调节。30名参与者被分为3组进行评估，包括10名ATLL患者、10名ACs和10名正常对照。从无任何化疗史的ATLL患者中采集血样。此外，还从ACs和正常个体中采集血样。对采集的血样进行白细胞分离。之后，从制备好的样本中提取RNA并用于cDNA合成。使用实时PCR研究作为病毒基因的HTLV-1碱性亮氨酸拉链因子以及包括、、、、、、、和在内的细胞基因。ATLL患者的平均年龄为53.2±7.32岁，9名（90%）为男性。与内对照相比，和的表达水平与ATLL患者显著相关。然而，其余组中其他基因表达的显著差异未被观察到。发现调节HTLV-1转化的病毒和细胞信号通路至关重要。可以考虑一种针对ATLL调节细胞信号通路的新治疗策略。因此，建议进行使用相关细胞信号通路激活剂和抑制剂对ATLL进行细胞治疗的临床试验。建议对和进行更多研究，以靶向这些基因并揭示ATLL的分子发病机制。
