College of Life Science, Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Fujian Provincial Universities Key Laboratory of Preventive Veterinary Medicine and Biotechnology (Longyan University), Longyan University, Longyan, Fujian, 364012, P.R. China.
Fujian Provincial Animal Husbandry Headquarters, Fuzhou, Fujian 350003, P.R. China.
Poult Sci. 2024 Sep;103(9):104032. doi: 10.1016/j.psj.2024.104032. Epub 2024 Jun 27.
Egg production is an important economic trait in layer ducks and understanding the genetics basis is important for their breeding. In this study, a genome-wide association study (GWAS) for egg production traits in 303 female Longyan Shan-ma ducks was performed based on a genotyping-by-sequencing strategy. Sixty-two single nucleotide polymorphisms (SNPs) associated with egg weight traits were identified (P < 9.48 × 10), including 8 SNPs at 5% linkage disequilibrium (LD)-based Bonferroni-corrected genome-wide significance level (P < 4.74 × 10). One hundred and nineteen SNPs were associated with egg number traits (P < 9.48 × 10), including 13 SNPs with 5% LD-based Bonferroni-corrected genome-wide significance (P < 4.74 × 10). These SNPs annotated 146 target genes which contained known candidate genes for egg production traits, such as prolactin and prolactin releasing hormone receptor. This study identified that these associated genes were significantly enriched in egg production-related pathways (P < 0.05), such as the oxytocin signaling, MAPK signaling, and calcium signaling pathways. It was notable that 18 genes were differentially expressed in ovarian tissues between higher and lower egg production in Shan-ma ducks. The identified potential candidate genes and pathways provide insight into the genetic basis underlying the egg production trait of layer ducks.
产蛋性能是蛋鸭重要的经济性状之一，解析其遗传基础对于蛋鸭的选育具有重要意义。本研究采用基于测序的基因分型策略，对 303 只龙岩山麻鸭的产蛋性能进行了全基因组关联分析（GWAS）。共鉴定到与蛋重性状相关的 62 个单核苷酸多态性（SNP）（P < 9.48 × 10-7），包括 5%连锁不平衡（LD）水平下基于 Bonferroni 校正的全基因组显著水平（P < 4.74 × 10-5）的 8 个 SNP；与产蛋数性状相关的 SNP 有 119 个（P < 9.48 × 10-7），包括 13 个 5% LD 水平下基于 Bonferroni 校正的全基因组显著水平（P < 4.74 × 10-5）的 SNP。这些 SNP 注释了 146 个靶基因，其中包含与产蛋性能相关的已知候选基因，如催乳素和催乳素释放激素受体。本研究表明，这些相关基因显著富集在与产蛋性能相关的途径中（P < 0.05），如催产素信号通路、MAPK 信号通路和钙信号通路。值得注意的是，在龙岩山麻鸭卵巢组织中，18 个基因在高、低产蛋鸭之间存在差异表达。鉴定到的潜在候选基因和通路为揭示蛋鸭产蛋性能的遗传基础提供了新的见解。
