Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Protein Expr Purif. 2024 Nov;223:106555. doi: 10.1016/j.pep.2024.106555. Epub 2024 Jul 14.
Whey, a valuable byproduct of dairy processing, contains essential proteins like β-lactoglobulin (βLG) and α-lactalbumin (αLA), making it a focus of research for its nutritional benefits. Various techniques, including chromatography and membrane filtration, are employed for protein extraction, often requiring multiple purification steps. One approach that has gained prominence for the purification and concentration of proteins, including those present in whey, is the use of polyethylene glycol (PEG) in aqueous two-phase systems. Our study simplifies this process by using PEG alone for whey protein purification. This approach yielded impressive results, achieving 92 % purity for βLG and 90 % for αLA. These findings underscore the effectiveness of PEG-based purification in isolating whey proteins with high purity.
乳清是乳制品加工的一种有价值的副产物，含有β-乳球蛋白（βLG）和α-乳白蛋白（αLA）等必需蛋白质，因此成为研究其营养价值的焦点。各种技术，包括色谱法和膜过滤法，都被用于蛋白质提取，通常需要多个纯化步骤。一种用于蛋白质（包括乳清中的蛋白质）纯化和浓缩的方法是在双水相系统中使用聚乙二醇（PEG）。我们的研究通过单独使用 PEG 来简化乳清蛋白的纯化过程。这种方法取得了令人瞩目的结果，βLG 的纯度达到 92%，αLA 的纯度达到 90%。这些发现强调了基于 PEG 的纯化在分离高纯度乳清蛋白方面的有效性。
