在廉价的锥形平底聚丙烯管中生长的大肠杆菌会产生高水平的 pUC 载体。
Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector.
机构信息
Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
Laboratory of Human Retrovirology and Immunoinformatics, Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA.
出版信息
J Microbiol Methods. 2024 Sep;224:106990. doi: 10.1016/j.mimet.2024.106990. Epub 2024 Jul 14.
Abstract
The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p < 0.001) than other PP. The use of the conical-PP can save research budgets in the current inflationary environment.
摘要
采用聚丙烯管（PP）从大肠杆菌中提取 pUC 衍生质粒，比较了圆管和锥形管之间的产量。在更便宜的平底锥形-PP 中生长的细胞的产量比其他 PP 高 1.5 倍（p<0.001）。在当前通货膨胀的环境下，使用锥形-PP 可以节省研究预算。
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