Dept. of Neuroscience and Rehabilitation, University of Ferrara, Ferrara 44121, Italy.
Dept. of Environmental and Prevention Sciences, University of Ferrara, Ferrara 44121, Italy.
Neurotoxicology. 2024 Sep;104:36-44. doi: 10.1016/j.neuro.2024.07.003. Epub 2024 Jul 14.
Ozone (O) forms in the Earth's atmosphere, both naturally and by reactions of man-made air pollutants. Deleterious effects of O have been found in the respiratory system. Here, we examine whether O alters olfactory behavior and cellular properties in the olfactory system. For this purpose, mice were exposed to O at a concentration found in highly polluted city air [0.8 ppm], and the behavior elicited by social and non-social odors in habituation/dishabituation tests was assessed. In addition, the electrical responses of dopaminergic olfactory bulb (OB) neurons were also evaluated. O differentially compromises olfactory perception to odors: it reduces responses to social and non-social odors in Swiss Webster mice, while this effect was observed in C57BL/6 J mice only for some non-social odors. Additionally, O reduced the rate of spontaneous spike firing in periglomerular dopaminergic cells (PG-DA) of the OB. Because this effect could reflect changes in excitability and/or synaptic inputs, the ability of O to alter PG-DA spontaneous activity was also tested together with cell membrane resistance, membrane potential, rheobase and chronaxie. Taken together, our data suggest the ability of O to affect olfactory perception.
臭氧（O）在地球大气中自然形成，并通过人为空气污染物的反应形成。已经发现 O 对呼吸系统有有害影响。在这里，我们研究了 O 是否会改变嗅觉系统中的嗅觉行为和细胞特性。为此，我们将小鼠暴露在浓度为城市高污染空气中的 O 中[0.8ppm]，并在习惯/去习惯测试中评估社交和非社交气味引起的行为。此外，还评估了多巴胺能嗅球（OB）神经元的电反应。O 对嗅觉感知产生不同的影响：它降低了瑞士 Webster 小鼠对社交和非社交气味的反应，而在 C57BL/6J 小鼠中，这种效应仅在某些非社交气味中观察到。此外，O 降低了 OB 中近球细胞多巴胺能细胞（PG-DA）的自发尖峰放电率。由于这种效应可能反映了兴奋性和/或突触输入的变化，因此还测试了 O 改变 PG-DA 自发活动的能力，以及细胞膜电阻、膜电位、阈值和弛豫时间。总之，我们的数据表明 O 有能力影响嗅觉感知。
