Chk2 同源物 Mek1 在酿酒酵母减数分裂重组过程中限制依赖于核酸外切酶 1 的 DNA 末端切除。
Chk2 homolog Mek1 limits exonuclease 1-dependent DNA end resection during meiotic recombination in Saccharomyces cerevisiae.
机构信息
Department of Radiation and Cellular Oncology/Department of Molecular Genetics and Cell Biology, University of Chicago, 920 E 58th Street, CLSC 817, Chicago, IL 60637, USA.
出版信息
Genetics. 2024 Sep 4;228(1). doi: 10.1093/genetics/iyae112.
Abstract
The conserved Rad2/XPG family 5'-3' exonuclease, exonuclease 1 (Exo1), plays many roles in DNA metabolism including during resolution of DNA double-strand breaks via homologous recombination. Prior studies provided evidence that the end resection activity of Exo1 is downregulated in yeast and mammals by Cdk1/2 family cyclin-dependent and checkpoint kinases, including budding yeast kinase Rad53 which functions in mitotic cells. Here, we provide evidence that the master meiotic kinase Mek1, a paralog of Rad53, limits 5'-3' single-strand resection at the sites of programmed meiotic DNA breaks. Mutational analysis suggests that the mechanism of Exo1 suppression by Mek1 differs from that of Rad53.
摘要
保守的 Rad2/XPG 家族 5'-3'外切核酸酶，外切核酸酶 1（Exo1），在 DNA 代谢中发挥着许多作用，包括通过同源重组来解决 DNA 双链断裂。先前的研究提供了证据，表明在酵母和哺乳动物中，Cdk1/2 家族细胞周期蛋白依赖性激酶和检查点激酶，包括在有丝分裂细胞中发挥作用的芽殖酵母激酶 Rad53，下调了 Exo1 的末端切除活性。在这里，我们提供的证据表明，主要的减数分裂激酶 Mek1，Rad53 的同源物，限制了有丝分裂 DNA 断裂部位的 5'-3'单链切除。突变分析表明，Mek1 对 Exo1 的抑制机制与 Rad53 不同。
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本文引用的文献
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