Li Yang, Zhao Meng-Jun, Lu Hong-Qiao, Liu Jian-Guo, Liu Yun-Kun, Wu Jia-Yuan
Hospital of Stomatology, Zunyi Medical University. Zunyi Guizhou 563000, Guizhou Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2024 Apr;33(2):164-169.
The characteristics of saliva and intestinal microbial community in children with high caries and no caries were analyzed by 16S rDNA high-throughput sequencing.
Among 431 children aged 3-5 years old in Zunyi City who were investigated previously by our team, 25 children in the high caries group and the same in the caries-free group were selected for fecal and saliva samples. 16S rDNA high-throughput sequencing was used to analyze the bacterial flora structure of the samples and identify the species with different relative abundance at the species level. SPSS 18.0 software package was used for data analysis.
The diversity of intestinal flora in the high caries group was higher than that in the caries-free group, and the difference was statistically significant(P＜0.05). The diversity of salivary flora in the high caries group was more than that in the caries-free group, with no significant difference(P＞0.05). At phylum level,there was no significant difference in intestinal and salivary flora between children with high caries and children without caries. At gene level, Blautia, [Eubacterium] hallii group and [Eubacterium] eligens group in the intestine of caries-free group were significantly higher than those of high caries group(P＜0.05), while Parasutterella and Christensenellaceae R-7 group were significantly lower than those of high caries group(P＜0.05). At gene level, Peptostreptococcus in saliva of caries-free group was significantly higher than that in high caries group(P＜0.05). Dialister, Kingella, Escherichia-Shigella and Treponema in saliva of caries-free group were significantly lower than those in high caries group(P＜0.05).
There are significant differences in species composition of intestinal flora but no in salivary flora between children with high caries and children without caries.
通过16S rDNA高通量测序分析高龋与无龋儿童唾液和肠道微生物群落特征。
在本团队前期调查的遵义市431名3 - 5岁儿童中，选取高龋组和无龋组各25名儿童采集粪便和唾液样本。采用16S rDNA高通量测序分析样本细菌菌群结构，鉴定物种水平上相对丰度不同的物种。使用SPSS 18.0软件包进行数据分析。
高龋组肠道菌群多样性高于无龋组，差异有统计学意义（P＜0.05）。高龋组唾液菌群多样性高于无龋组，但差异无统计学意义（P＞0.05）。在门水平上，高龋儿童与无龋儿童的肠道和唾液菌群无显著差异。在属水平上，无龋组肠道中的布劳特氏菌属、哈氏真杆菌群和 eligens 真杆菌群显著高于高龋组（P＜0.05），而副萨特氏菌属和克里斯滕森菌科R - 7群显著低于高龋组（P＜0.05）。在属水平上，无龋组唾液中的消化链球菌显著高于高龋组（P＜0.05）。无龋组唾液中的戴阿利斯特菌属、金氏菌属、大肠埃希菌 - 志贺菌属和密螺旋体属显著低于高龋组（P＜0.05）。
高龋儿童与无龋儿童肠道菌群物种组成存在显著差异，而唾液菌群无显著差异。
