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碳免疫测定法——一种用于猫弓形虫病的简单快速血清诊断测试。

Carbon immunoassay--a simple and rapid serodiagnostic test for feline toxoplasmosis.

作者信息

Pakes S P, Lai W C

出版信息

Lab Anim Sci. 1985 Aug;35(4):370-2.

PMID:3900579
Abstract

A serodiagnostic test, simpler and more rapid to perform than traditional methods, was sought to identify Toxoplasma gondii antibody in research cats. The reliability and sensitivity of the direct and indirect carbon immunoassay (CIA) tests were compared to each other and to the indirect immunofluorescent antibody (IFA) test. The three tests were used to detect the presence or absence of T. gondii antibody in the sera of 94 cats. The results of this study show that the CIA tests correlate with one another and the IFA test nearly 99%, indicating they are highly reliable. Comparison of titers of the positive sera indicate a high degree of sensitivity as well.

摘要

人们一直在寻找一种血清诊断测试方法,它比传统方法更简单、执行速度更快,用于识别研究用猫体内的弓形虫抗体。将直接和间接碳免疫测定(CIA)测试的可靠性和灵敏度相互比较,并与间接免疫荧光抗体(IFA)测试进行比较。这三种测试被用于检测94只猫血清中弓形虫抗体的存在与否。这项研究的结果表明,CIA测试之间以及与IFA测试的相关性接近99%,表明它们高度可靠。对阳性血清滴度的比较也表明其具有高度的灵敏度。

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