基于16S rRNA基因测序的肌肉减少症患者肠道微生物群变化：一项系统综述和荟萃分析
Changes in the gut microbiota of patients with sarcopenia based on 16S rRNA gene sequencing: a systematic review and meta-analysis.
作者信息
Song Qi, Zhu Youkang, Liu Xiao, Liu Hai, Zhao Xinyi, Xue Liyun, Yang Shaoying, Wang Yujia, Liu Xifang
机构信息
Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.
Xi'an Physical Education University, Xi'an, China.
出版信息
Front Nutr. 2024 Jun 28;11:1429242. doi: 10.3389/fnut.2024.1429242. eCollection 2024.
INTRODUCTION
Sarcopenia, an age-related disease, has become a major public health concern, threatening muscle health and daily functioning in older adults around the world. Changes in the gut microbiota can affect skeletal muscle metabolism, but the exact association is unclear. The richness of gut microbiota refers to the number of different species in a sample, while diversity not only considers the number of species but also the evenness of their abundances. Alpha diversity is a comprehensive metric that measures both the number of different species (richness) and the evenness of their abundances, thereby providing a thorough understanding of the species composition and structure of a community.
METHODS
This meta-analysis explored the differences in intestinal microbiota diversity and richness between populations with sarcopenia and non-sarcopenia based on 16 s rRNA gene sequencing and identified new targets for the prevention and treatment of sarcopenia. PubMed, Embase, Web of Science, and Google Scholar databases were searched for cross-sectional studies on the differences in gut microbiota between sarcopenia and non-sarcopenia published from 1995 to September 2023 scale and funnel plot analysis assessed the risk of bias, and performed a meta-analysis with State v.15. 1.
RESULTS
A total of 17 randomized controlled studies were included, involving 4,307 participants aged 43 to 87 years. The alpha diversity of intestinal flora in the sarcopenia group was significantly reduced compared to the non-sarcopenia group: At the richness level, the proportion of Actinobacteria and Fusobacteria decreased, although there was no significant change in other phyla. At the genus level, the abundance of f-Ruminococcaceae; g-, g-, , and other genera decreased, whereas the abundance of g-, , and increased.
DISCUSSION
This study showed that the richness of the gut microbiota decreased with age in patients with sarcopenia. Furthermore, the relative abundance of different microbiota changed related to age, comorbidity, participation in protein metabolism, and other factors. This study provides new ideas for targeting the gut microbiota for the prevention and treatment of sarcopenia.
SYSTEMATIC REVIEW REGISTRATION
https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=475887, CRD475887.
引言
肌肉减少症是一种与年龄相关的疾病，已成为一个主要的公共卫生问题，威胁着全球老年人的肌肉健康和日常功能。肠道微生物群的变化会影响骨骼肌代谢，但其确切关联尚不清楚。肠道微生物群的丰富度是指样本中不同物种的数量，而多样性不仅考虑物种数量，还考虑其丰度的均匀度。α多样性是一种综合指标，它既衡量不同物种的数量（丰富度），也衡量其丰度的均匀度，从而全面了解群落的物种组成和结构。
方法
本荟萃分析基于16s rRNA基因测序探讨了肌肉减少症患者与非肌肉减少症患者肠道微生物群多样性和丰富度的差异，并确定了预防和治疗肌肉减少症的新靶点。检索了PubMed、Embase、Web of Science和谷歌学术数据库中1995年至2023年9月发表的关于肌肉减少症与非肌肉减少症患者肠道微生物群差异的横断面研究，并通过漏斗图分析评估偏倚风险，使用State v.15.1进行荟萃分析。
结果
共纳入17项随机对照研究，涉及4307名年龄在43至87岁之间的参与者。与非肌肉减少症组相比，肌肉减少症组肠道菌群的α多样性显著降低：在丰富度水平上，放线菌门和梭杆菌门的比例下降，但其他门没有显著变化。在属水平上，f-瘤胃球菌科、g-、g-、等属的丰度下降，而g-、、等属的丰度增加。
讨论
本研究表明肌肉减少症患者肠道微生物群的丰富度随年龄下降。此外，不同微生物群的相对丰度因年龄、合并症、参与蛋白质代谢等因素而发生变化。本研究为针对肠道微生物群预防和治疗肌肉减少症提供了新思路。
系统评价注册
https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=475887, CRD475887
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