Kawai Michio, Oda Haruka, Mimura Hisatoshi, Osaki Toshihisa, Takeuchi Shoji
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, Tokyo, Japan.
Artificial Cell Membrane Systems Group, Kanagawa Institute of Industrial Science and Technology, Kanagawa, Japan.
HardwareX. 2024 Jun 15;19:e00545. doi: 10.1016/j.ohx.2024.e00545. eCollection 2024 Sep.
The development of a compact and affordable fluorescence microscope can be a formidable challenge for growing needs in on-site testing and detection of fluorescent labeled biological systems, especially for those who specialize in biology rather than in engineering. In response to such a situation, we present an open-source miniature fluorescence microscope using Raspberry Pi. Our fluorescence microscope, with dimensions of 19.2 × 13.6 × 8.2 cm (including the display, computer, light-blocking case, and other operational requirements), not only offers cost-effectiveness (costing less than $500) but is also highly customizable to meet specific application needs. The 12.3-megapixel Raspberry Pi HQ Camera captures high-resolution imagery, while the equipped wide-angle lens provides a field of view measuring 21 × 15 mm. The integrated wireless LAN in the Raspberry Pi, along with software-controllable high-powered fluorescence LEDs, holds potential for a wide range of applications. This open-source fluorescence microscope offers biohybrid sensor developers a versatile tool to streamline unfamiliar mechanical design tasks and open new opportunities for on-site fluorescence detections.
对于现场检测和荧光标记生物系统的需求不断增长而言，开发一款紧凑且经济实惠的荧光显微镜可能是一项艰巨的挑战，尤其是对于那些专门从事生物学而非工程学的人来说。针对这种情况，我们展示了一款使用树莓派的开源微型荧光显微镜。我们的荧光显微镜尺寸为19.2×13.6×8.2厘米（包括显示屏、计算机、遮光箱和其他操作要求），不仅具有成本效益（成本低于500美元），而且还高度可定制，以满足特定的应用需求。1230万像素的树莓派HQ摄像头可捕捉高分辨率图像，而配备的广角镜头提供21×15毫米的视野。树莓派中集成的无线局域网，以及软件可控的高功率荧光LED，具有广泛的应用潜力。这款开源荧光显微镜为生物混合传感器开发者提供了一个多功能工具，以简化不熟悉的机械设计任务，并为现场荧光检测开辟新机会。
