Department of Materials Science and Nanotechnology, Graduate School of Natural and Applied Sciences, Konya Food and Agriculture University, 42080 Konya, Turkey.
Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, 42080 Konya, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123222. doi: 10.1016/j.saa.2023.123222. Epub 2023 Jul 31.
Herein, we report fluorescein-labelled silica nanoparticles (FSNP) which serve as fluorescent nano-chemosensors for sequential detection of Pb (which is a toxic heavy metal) and dipicolinic acid (DPA) (which is a distinctive indicator biomarker of bacterial spores) with high sensitivity and selectivity. The fluorescence of FSNP is quenched because of the complex formation between Pb ions and surface amide groups, however, the fluorescence is recovered in contact with DPA, resulting from the association of DPA with surface bound Pb ions. FSNP-Pb complexes show high sensitivity towards DPA with a low detection limit of 850 nM which is approximately seventy times lower than the infectious dosage of bacterial spores (60 μM). Lateral flow test platform was further developed to show the applicability and practicability of our system.
在这里，我们报告了荧光素标记的二氧化硅纳米粒子（FSNP），它们可以作为荧光纳米化学传感器，用于对 Pb（一种有毒重金属）和二吡啶甲酸（DPA）（细菌孢子的独特指示生物标志物）进行顺序检测，具有高灵敏度和选择性。由于 Pb 离子与表面酰胺基团形成配合物，FSNP 的荧光被猝灭，但是，与 DPA 接触时，荧光会恢复，这是由于 DPA 与表面结合的 Pb 离子结合所致。FSNP-Pb 配合物对 DPA 表现出高灵敏度，检测限低至 850 nM，大约比细菌孢子的感染剂量（60 μM）低七十倍。进一步开发了横向流动测试平台，以展示我们系统的适用性和实用性。
