Visconti Gioele, de Figueiredo Miguel, Monnier Joanie, Shea Julia, Rudaz Serge, Glauser Gaetan
School of Pharmaceutical Sciences, University of Geneva, CMU - Rue Michel-Servet 1, Geneva, Switzerland.
Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU - Rue Michel-Servet 1, Geneva, Switzerland.
Food Chem X. 2024 Jun 17;23:101565. doi: 10.1016/j.fochx.2024.101565. eCollection 2024 Oct 30.
Neonicotinoids, a highly effective class of insecticides used worldwide, have been identified as a major cause of concern for biodiversity. To assess the ecological and environmental consequences of neonicotinoids' use, reliable analytical methodologies, including calibration approaches, are needed. Here, we compared the performance of internal calibration (IC) using a single concentration of stable isotope-labeled standard (SIL) with classical multipoint external calibration (EC) for the quantification of six neonicotinoids in honey. IC showed acceptable levels of trueness (86.3% - 116.0%) and precision (1.4% - 20.8%), although slight biases were observed at very low concentrations compared to EC. When applied to 32 original honey samples, both approaches showed strong agreement (R > 0.998) with proportional biases lower than 5%. These results highlight the possibility of implementing IC to simplify quantification in liquid chromatography-mass spectrometry-based pesticide applications.
新烟碱类杀虫剂是一类在全球广泛使用的高效杀虫剂，已被确定为生物多样性的一个主要担忧因素。为了评估使用新烟碱类杀虫剂的生态和环境后果，需要可靠的分析方法，包括校准方法。在这里，我们比较了使用单一浓度稳定同位素标记标准品（SIL）的内标法（IC）与经典多点外标法（EC）对蜂蜜中六种新烟碱类杀虫剂进行定量分析的性能。内标法显示出可接受的准确度水平（86.3% - 116.0%）和精密度水平（1.4% - 20.8%），尽管与外标法相比，在极低浓度下观察到了轻微偏差。当应用于32个原始蜂蜜样品时，两种方法都显示出很强的一致性（R > 0.998），比例偏差低于5%。这些结果突出了在内标法在基于液相色谱 - 质谱的农药应用中简化定量分析的可能性。
