检测 HIV 感染女性宫颈阴道灌洗液中的细胞因子及其与高危型人乳头瘤病毒的关系。

Detection of cytokines in cervicovaginal lavage in HIV-infected women and its association with high-risk human papillomavirus.

机构信息

Programa de Pós-graduação em Medicina e Saúde (PPgMS), Universidade Federal da Bahia, Salvador, Brazil.

Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil.

出版信息

Front Immunol. 2024 Jun 28;15:1416204. doi: 10.3389/fimmu.2024.1416204. eCollection 2024.

DOI:10.3389/fimmu.2024.1416204

PMID:39007140

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11239429/

Abstract

BACKGROUND

Women living with HIV/AIDS (WLHA) have an increased prevalence of high-risk HPV infection (HR-HPV) and cervical intraepithelial neoplasia (CIN) and a greater risk of cervical cancer despite access to a new generation of antiretroviral therapy. The aim of this study is to evaluate the concentrations of different cytokines involved in the local immune response in WLHA, which is fundamental for understanding the pathogenesis of HPV-related cancer in this population.

METHODS

IL-1β, IL-2, IL-4, IL-6, IL-10, IFN-γ, TNF-α, IP-10, GM-CSF, and MIP-1α were investigated in the cervicovaginal lavage (CVL) of 106 WLHA attending at Hospital Universitario Professor Edgard Santos in Salvador, Bahia, Brazil, during the period December 2019 to April 2023 by Luminex. All participants were also tested for and and underwent colposcopy, Pap smear, and Nugent score. HIV plasma viral load (VL) and CD4 cell count were performed for all WLHA.

RESULTS

In this study, 22.6% (24/106) of WLHA were infected with HR-HPV. A higher proportion of patients with HR-HPV (66.7%) had detectable levels of IL-10 than those negative ones (40.2%, = 0.02). More premenopausal women had either IL-6 (51.4%) or IP-10 (58.3%) than those in menopausal status (26.5% for IL-6 and 32.4% for IP-10, = 0.013 and = 0.011, respectively). Vaginosis was negatively associated with detection of IP-10 (24.2% vs. 61.4%, < 0.001) and INF-γ (39.4% vs. 68.6%, = 0.005). A positive association was detected for IL-1β (66.7 vs. 37.1%, = 0.005) and IL-10 (63.6% vs. 37.1%, = 0.01). VL and CD4 were not associated with the studied cytokines.

CONCLUSION

We demonstrated a positive association between IL-10 and HPV infection in CVL, suggesting the predominance of the Th2 response in HIV/HPV co-infected patients. However, further studies with longer follow-up will be needed to evaluate the association of IL-10 with HPV infection, CIN, and cervical cancer in WLHA.

摘要

背景

尽管可以获得新一代抗逆转录病毒疗法，但患有艾滋病毒/艾滋病的妇女（WLHA）的高危人乳头瘤病毒（HR-HPV）感染和宫颈上皮内瘤变（CIN）的患病率更高，宫颈癌的风险也更高。本研究旨在评估参与巴西萨尔瓦多教授埃德加·桑托斯大学医院的 106 名 WLHA 的宫颈阴道灌洗液（CVL）中涉及局部免疫反应的不同细胞因子的浓度，这对于理解该人群中 HPV 相关癌症的发病机制至关重要。

方法

通过 Luminex 检测 2019 年 12 月至 2023 年 4 月期间在巴西萨尔瓦多教授埃德加·桑托斯大学医院就诊的 106 名 WLHA 的 CVL 中 IL-1β、IL-2、IL-4、IL-6、IL-10、IFN-γ、TNF-α、IP-10、GM-CSF 和 MIP-1α。所有参与者还接受了和的检测，并接受了阴道镜检查、巴氏涂片检查和 Nugent 评分。对所有 WLHA 进行 HIV 血浆病毒载量（VL）和 CD4 细胞计数。

结果

在这项研究中，22.6%（24/106）的 WLHA 感染了 HR-HPV。与阴性者相比，感染 HR-HPV 的患者（66.7%）具有更高水平的 IL-10（40.2%， = 0.02）。更多的绝经前妇女有 IL-6（51.4%）或 IP-10（58.3%），而绝经后妇女有 IL-6（26.5%）和 IP-10（32.4%）， = 0.013 和 = 0.011，分别）。阴道病与 IP-10 的检测呈负相关（24.2%对 61.4%， < 0.001）和 INF-γ（39.4%对 68.6%， = 0.005）。IL-1β（66.7%对 37.1%， = 0.005）和 IL-10（63.6%对 37.1%， = 0.01）的检测呈正相关。VL 和 CD4 与研究中的细胞因子无关。