UMR Transfronalière BioEcoAgro-INRae 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d'Opale, F-59000 Lille, France.
Ingredia S.A., 51 Av. Lobbedez - CS 60946, 62033 Arras Cedex, France.
Food Funct. 2024 Jul 29;15(15):8104-8115. doi: 10.1039/d4fo01637h.
Calcium is the most abundant mineral in the human body and is involved in critical physiological and cellular processes. It is essential for the development, maintenance, and integrity of bone tissue throughout life. Identifying new natural food-grade chelating agents to improve calcium uptake is of increasing interest. Casein phosphopeptides (CPPs), highly phosphorylated peptides obtained after enzymatic hydrolysis of caseins, represent promising calcium-chelating candidates. The aim of this study was to investigate, using cell culture models, the ability of a digested milk matrix enriched in CPPs to regulate calcium transport through the intestinal barrier and elucidate the involved mechanisms. To this end, a CPP-preparation underwent static digestion and was subsequently incubated with an intestinal barrier model to monitor calcium uptake and transport. Our results demonstrated that the digested CPP preparation enhanced the -epithelial calcium transport paracellular pathways and that CPPs, identified by peptidomics, crossed the intestinal barrier in the same time.
钙是人体中含量最丰富的矿物质，参与关键的生理和细胞过程。它对于骨骼组织的发育、维持和完整至关重要。因此，寻找新的天然食品级螯合剂以提高钙的吸收利用率引起了人们越来越多的关注。酪蛋白磷酸肽（CPPs）是经过酶解酪蛋白得到的高度磷酸化肽，是很有前途的钙螯合剂候选物。本研究旨在使用细胞培养模型，研究富含 CPP 的消化牛奶基质调控肠道屏障钙转运的能力，并阐明相关机制。为此，对 CPP 制剂进行静态消化，然后与肠道屏障模型孵育，以监测钙摄取和转运。结果表明，消化后的 CPP 制剂增强了钙的 -上皮细胞旁途径转运，并且通过肽组学鉴定出的 CPP 也在同一时间穿过了肠道屏障。
