School of Biomedical Sciences and Engineering, South China University of Technology, Guangzhou International Campus, Guangzhou, Guangdong 511442, People's Republic of China.
Center for Medical Research on Innovation and Translation, Institute of Clinical Medicine, Guangzhou First People's Hospital, the Second Affiliated Hospital, South China University of Technology, Guangzhou, Guangdong 510180, People's Republic of China.
Nano Lett. 2024 Jul 24;24(29):9017-9026. doi: 10.1021/acs.nanolett.4c02113. Epub 2024 Jul 15.
The development of in situ tumor vaccines offers promising prospects for cancer treatment. Nonetheless, the generation of plenary autologous antigens in vivo and their codelivery to DC cells along with adjuvants remains a significant challenge. Herein, we developed an in situ tumor vaccine using a supramolecular nanoparticle/hydrogel composite (ANP/ALCD) and a deformable nanoadjuvant (PPE). The ANP/ALCD composite consisted of β-cyclodextrin-decorated alginate (Alg--CD) and MTO-encapsulated adamantane-decorated nanoparticles (ANP) through supramolecular interaction, facilitating the long-term and sustained production of plenary autologous antigens, particularly under a 660 nm laser. Simultaneously, the produced autologous antigens were effectively captured by nanoadjuvant PPE and subsequently transported to lymph nodes and DC cells, benefiting from its optimized size and deformability. This in situ tumor vaccine can trigger a robust antitumor immune response and demonstrate significant therapeutic efficacy in inhibiting tumor growth, suppressing tumor metastasis, and preventing postoperative recurrence, offering a straightforward approach to programming in situ tumor vaccines.
原位肿瘤疫苗的发展为癌症治疗提供了有前景的方案。然而，在体内生成完全自体抗原并将其与佐剂一起递送至 DC 细胞仍然是一个重大挑战。在此，我们开发了一种使用超分子纳米粒子/水凝胶复合材料（ANP/ALCD）和可变形纳米佐剂（PPE）的原位肿瘤疫苗。ANP/ALCD 复合材料由通过超分子相互作用修饰的β-环糊精海藻酸钠（Alg--CD）和封装 MTO 的金刚烷修饰纳米粒子（ANP）组成，有利于长期和持续产生完全自体抗原，特别是在 660nm 激光下。同时，所产生的自体抗原被纳米佐剂 PPE 有效捕获，并随后运送到淋巴结和 DC 细胞，这得益于其优化的尺寸和变形性。这种原位肿瘤疫苗可以引发强烈的抗肿瘤免疫反应，并在抑制肿瘤生长、抑制肿瘤转移和预防术后复发方面表现出显著的治疗效果，为原位肿瘤疫苗的编程提供了一种简单的方法。
