Walsh F S, Moore S E
Neurosci Lett. 1985 Aug 16;59(1):73-8. doi: 10.1016/0304-3940(85)90217-4.
Antibody to the cell adhesion molecule N-CAM was used for analysis of cryostat sections using indirect immunofluorescence assay of human muscle biopsies from patients with a variety of muscle diseases. Positive staining was restricted to cases where haematoxylin and eosin staining revealed regenerating myofibers. N-CAM reactivity was specifically found associated with such regenerating fibres. No staining was found on normal adult, degenerating or necrotic fibres. In addition, in chronically denervated muscle disease there was no staining of either atrophic fibres or reinnervated fibres that tended to exist in fibre type specific groupings. N-CAM can therefore be regarded as a specific gene product of regenerating myofibres and is an additional marker of these fibres on biopsy of human muscle.
利用针对细胞黏附分子N-CAM的抗体，通过间接免疫荧光法对来自患有各种肌肉疾病患者的人体肌肉活检组织的低温切片进行分析。阳性染色仅限于苏木精和伊红染色显示有再生肌纤维的病例。N-CAM反应性特别与这类再生纤维相关。在正常成人、变性或坏死纤维上未发现染色。此外，在慢性失神经支配的肌肉疾病中，萎缩纤维或倾向于以纤维类型特异性分组存在的再支配纤维均未染色。因此，N-CAM可被视为再生肌纤维的一种特异性基因产物，并且是人体肌肉活检时这些纤维的一种额外标志物。
