Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo CruzRio de Janeiro, Brazil.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2024 Sep;41(9):1158-1170. doi: 10.1080/19440049.2024.2376156. Epub 2024 Jul 15.
This study aimed to develop and validate a multi-mycotoxin analysis method applied to cashew nuts by employing a miniaturized QuEChERS method followed by determination by ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Satisfactory recoveries for the concentrations 1, 10 and 30 ng g, ranging from 66% (fumonisin B) to 110% (ochratoxin A) and relative standard deviations lower than 9% (fumonisin B) were obtained for the target compounds. Limits of quantification ranged from 0.004 ng g (sterigmatocystin) to 0.59 ng g (alternariol). The applicability of the analytical method was verified by analyzing 30 cashew nut samples from the city of Rio de Janeiro, RJ, southeastern Brazil. Aflatoxins M1, G2, G1, B2, B1, ochratoxin A and sterigmatocystin were detected, respectively, in 27%, 10%, 17%, 30%, 30%, 30% and 50% of the analyzed samples, at maximum concentrations of 0.56, 0.67, 1.43, 2.02, 4.93, 4.81, and 0.35 ng g. The maximum limit established by Brazilian legislation for aflatoxins was not exceeded by any of the analyzed samples.
本研究旨在开发和验证一种应用于腰果的多真菌毒素分析方法，采用微型 QuEChERS 方法，随后通过超高效液相色谱串联质谱法（UHPLC-MS/MS）进行测定。对于浓度为 1、10 和 30ng/g 的目标化合物，回收率令人满意，范围为 66%（伏马菌素 B）至 110%（赭曲霉毒素 A），相对标准偏差低于 9%（伏马菌素 B）。定量限范围为 0.004ng/g（桔青霉素）至 0.59ng/g（交链孢酚）。该分析方法的适用性通过分析来自巴西东南部里约热内卢市的 30 个腰果样本得到了验证。分别在 27%、10%、17%、30%、30%、30%和 50%的分析样本中检测到黄曲霉毒素 M1、G2、G1、B2、B1、赭曲霉毒素 A 和桔青霉素，其最大浓度分别为 0.56、0.67、1.43、2.02、4.93、4.81 和 0.35ng/g。分析样本均未超过巴西法规规定的黄曲霉毒素最大限量。
