Pretreatment of biomass with ethanol/deep eutectic solvent towards higher component recovery and obtaining lignin with high β-O-4 content.

Deep eutectic solvent (DES) is an ideal solvent for extracting lignin in biomass pretreatment process. However, excessive breakage of the β-O-4 bonds of lignin remained a challenge for DES-pretreated biomass. In this study, a novel pretreatment system of choline chloride-citrate acid DES combined with ethanol for the pretreatment of bamboo was developed. The chemical characteristics of extracted lignin of bamboo before and after pretreatment were analyzed by gel permeation chromatography (GPC) and nuclear magnetic resonance spectroscopy (NMR). The results showed that the lignin extracted by ethanol/DES had moderate and uniform molecular weight (M: 3081-4314 Da, M: 3130-5399 Da), and was structurally intact (maintaining 40.29 % β-O-4 content), which was about five times higher than DES-extracted lignin, and contained a high number of S units (up to 80 %). Ethanol/DES system resulted in high removal of lignin up to 78.81 % and the highest enzymatic digestibility of glucose (72.68 %) and xylan (92.95 %), respectively. In addition, recovered DES provided similar glucose digestibility yields and delignification performance. The Ethanol/DES pretreatment developed herein provided a viable method for maintaining the structural integrity of lignin and preparing lignin with high β-O-4 content whilst with a relatively high components recovery.