Helk B, Sprinzl M
Nucleic Acids Res. 1985 Sep 11;13(17):6283-98. doi: 10.1093/nar/13.17.6283.
Fragments of tRNA possessing a free TpsiC-loop or a free D-loop form stable complexes with the colicin fragment (1494-1542) of 16S ribosomal RNA from E. coli. The colicin fragment does not bind to tRNA in which the T-loop and the D-loop are involved in tertiary interactions. Colicin cleavage of the 16S rRNA from E. coli is inhibited by aminoacyl-tRNA or tRNA fragments, indicating that a similar interaction may take place on the intact 70S ribosomes. The oligonucleotide d(G-T-T-C-G-A)homologous to the conserved sequence G-T-psi-C-Pu-(m1)A in the TpsiC-region of many elongator tRNAs binds to the conserved sequence U-C-G-mU-A-A-C (1495-1501) of the 16S rRNA. It is suggested that the 3'-end of the 16S rRNA may provide the part of the binding site for the elongator tRNAs on bacterial ribosomes.
拥有游离TψC环或游离D环的tRNA片段与来自大肠杆菌的16S核糖体RNA的大肠杆菌素片段（1494 - 1542）形成稳定复合物。大肠杆菌素片段不与T环和D环参与三级相互作用的tRNA结合。氨酰tRNA或tRNA片段可抑制大肠杆菌16S rRNA的大肠杆菌素切割，这表明在完整的70S核糖体上可能发生类似的相互作用。与许多延伸tRNA的TψC区域中保守序列G-T-ψC-Pu-(m1)A同源的寡核苷酸d(G-T-T-C-G-A)与16S rRNA的保守序列U-C-G-mU-A-A-C（1495 - 1501）结合。有人提出，16S rRNA的3'端可能为细菌核糖体上延伸tRNA的结合位点提供部分区域。
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