蛋白质合成不需要与tRNA互补的保守5S rRNA。
Conserved 5S rRNA complement to tRNA is not required for protein synthesis.
作者信息
Pace B, Matthews E A, Johnson K D, Cantor C R, Pace N R
出版信息
Proc Natl Acad Sci U S A. 1982 Jan;79(1):36-40. doi: 10.1073/pnas.79.1.36.
Abstract
The notion that tRNA and 5S rRNA interact through evolutionarily conserved complementary sequences has been tested by nucleolytic modification of the 5S rRNA, using the modified rRNA to reconstitute the large ribosomal subunit, and assaying for poly(uridylic acid)-directed polyphenylalanine synthesis. The 5S rRNA sequence C-G-A-A (residues 43-46) and several residues surrounding it are not essential for protein synthesis.
摘要
tRNA与5S rRNA通过进化上保守的互补序列相互作用这一观点，已通过对5S rRNA进行核酸酶修饰来进行验证，即使用修饰后的rRNA重建大核糖体亚基，并检测聚（尿苷酸）指导的聚苯丙氨酸合成。5S rRNA序列C-G-A-A（第43 - 46位残基）及其周围的几个残基对蛋白质合成并非必需。
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