DeLozanne A, Lewis M, Spudich J A, Leinwand L A
Proc Natl Acad Sci U S A. 1985 Oct;82(20):6807-10. doi: 10.1073/pnas.82.20.6807.
Despite many biochemical and structural similarities between muscle and nonmuscle myosins, their genes appear to have completely diverged, since muscle myosin molecular clones will not hybridize to RNA from nonmuscle sources. Here we report the isolation and characterization of a partial myosin heavy chain (MHC) cDNA clone from the slime mold Dictyostelium discoideum. We have isolated this clone from a lambda gt11 expression cDNA library by antibody screening. In contrast to the highly conserved sarcomeric muscle MHC multigene families in other organisms, there appears to be only one gene encoding MHC in the Dictyostelium genome. The cloned portion of this gene does not hybridize to the genomic DNAs of other eukaryotic organisms. Analysis of the predicted amino acid sequence of the partial Dictyostelium MHC clone shows that while there is no sequence homology to known striated muscle MHCs, the structure- and coiled-coil-forming capacities have been conserved.
尽管肌肉肌球蛋白和非肌肉肌球蛋白在生化和结构上有许多相似之处，但它们的基因似乎已经完全分化，因为肌肉肌球蛋白分子克隆不会与来自非肌肉来源的RNA杂交。在这里，我们报告了从黏菌盘基网柄菌中分离和鉴定部分肌球蛋白重链（MHC）cDNA克隆的情况。我们通过抗体筛选从λgt11表达cDNA文库中分离出了这个克隆。与其他生物中高度保守的肌节肌肉MHC多基因家族不同，盘基网柄菌基因组中似乎只有一个编码MHC的基因。该基因的克隆部分不会与其他真核生物的基因组DNA杂交。对部分盘基网柄菌MHC克隆预测氨基酸序列的分析表明，虽然与已知的横纹肌MHC没有序列同源性，但结构和形成卷曲螺旋的能力得以保留。
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