Rozek C E, Davidson N
Cell. 1983 Jan;32(1):23-34. doi: 10.1016/0092-8674(83)90493-2.
We have isolated overlapping genomic clones that contain a single-copy myosin heavy-chain gene of Drosophila melanogaster. By the criteria of hybridization under nonstringent conditions, this is the only myosin heavy-chain gene of the fly. The gene is localized to the chromosomal region 36B. The transcriptional unit has a length of about 19 kb and contains at least nine introns. There are three mature mRNA species expressed from the gene, with molecular lengths of 7.2 kb, 8.0 kb and 8.6 kb. The former two are expressed abundantly during those stages of development when larval muscle proteins are being synthesized; the latter two are expressed when the principal muscle proteins of the adult are made. The three transcripts differ primarily in their respective patterns of splicing at the 3' ends.
我们分离出了重叠的基因组克隆，这些克隆包含果蝇单拷贝的肌球蛋白重链基因。根据在非严格条件下杂交的标准，这是果蝇唯一的肌球蛋白重链基因。该基因定位于染色体区域36B。转录单元长度约为19 kb，至少包含9个内含子。该基因表达出三种成熟的mRNA，分子长度分别为7.2 kb、8.0 kb和8.6 kb。前两种在幼虫肌肉蛋白合成的发育阶段大量表达；后两种在成虫主要肌肉蛋白合成时表达。这三种转录本的主要差异在于其3'端各自的剪接模式。
