College of Dental Medicine, University of New England.
Adams School of Dentistry, University of North Carolina at Chapel Hill.
J Oral Sci. 2024;66(3):163-168. doi: 10.2334/josnusd.24-0031.
Using X-ray micro-computed tomography (micro-CT), the aim of this study was to measure the porosity of two tricalcium silicate sealers (EndoSequence BC and NeoSealer Flo) applied using three obturation techniques (single-cone, warm-vertical, and cold-lateral) to six single-rooted human teeth.
Six extracted, single-rooted human teeth were shaped with ProTaper Next rotary files and obturated with EndoSequence BC or NeoSealer Flo sealers and gutta-percha (GP) using one of the three techniques above. Micro-CT was used to map the full length of the canals. Deep learning cross-sectional segmentation was used to analyze image slices of the apical (0-2 mm) and coronal (14-16 mm from the apex) regions (n = 230-261 per tooth) for the areas of GP and sealer, as well as porosity. Median (%) with interquartile range of porosity were calculated , and the results were statistically analyzed with the Kruskal-Wallis test.
In the apical region, EndoSequence BC had significantly fewer pores than NeoSealer Flo with the single-cone obturation (% median-interquartile range, IQR: 0.00-1.62) and warm-vertical condensation (5.57-10.32) techniques, whereas in the coronal region, NeoSealer Flo had significantly fewer pores than EndoSequence BC with these two techniques (0.39-5.02) and (0.10-0.19), respectively. There was no significant difference in porosity between the two sealers for the cold-lateral condensation technique in both the apical and coronal regions.
For optimal obturation, the choice of technique and sealer is critical.
本研究使用 X 射线微计算机断层扫描(micro-CT)测量两种硅酸三钙密封剂(EndoSequence BC 和 NeoSealer Flo)的孔隙率,这两种密封剂分别采用三种根管充填技术(单根管、热垂直、冷侧方)充填到六颗人单根牙中。
六颗离体的、单根的人牙使用 ProTaper Next 旋转锉预备,并使用上述三种技术中的一种分别用 EndoSequence BC 或 NeoSealer Flo 密封剂和牙胶(GP)充填。微 CT 用于对整个根管长度进行成像。使用深度学习的横截面分割方法对根尖(距根尖 0-2mm)和冠部(距根尖 14-16mm)区域的图像切片(每个牙 230-261 个切片)进行分析,以确定 GP 和密封剂的面积以及孔隙率。计算每个牙的根尖(0-2mm)和冠部(14-16mm 距根尖)区域的 GP 和密封剂的中位数(%)及四分位距(IQR),并用 Kruskal-Wallis 检验对结果进行统计学分析。
在根尖区域,与单根管充填和热垂直充填技术相比,EndoSequence BC 的孔隙率显著低于 NeoSealer Flo(%中位数-四分位距,IQR:0.00-1.62),而在冠部区域,与这两种技术相比,NeoSealer Flo 的孔隙率显著低于 EndoSequence BC(0.39-5.02)和(0.10-0.19)。在根尖和冠部区域,冷侧方充填技术的两种密封剂的孔隙率没有显著差异。
为了获得最佳的根管充填效果,选择合适的技术和密封剂至关重要。