Department of Biological and Agriculture Science, Federal University of Espírito Santo, São Mateus, ES, Brazil.
Department of Agronomy, Federal University of the Jequitinhonha and Mucuri Valleys, Diamantina, Minas Gerais, Brazil.
Int J Phytoremediation. 2024 Nov;26(13):2175-2182. doi: 10.1080/15226514.2024.2379603. Epub 2024 Jul 15.
The objective of this study was to determine the efficiency of the microbial rhizosphere () in the phytoremediation of sulfentrazone using quantification methods (CO2 evolution, microbial biomass carbon, and metabolic quotient) and identification of bacteria (PCR-DGGE technique). The experiment was conducted in a completely randomized design, in a 2x4 factorial scheme, with four replications. The treatments were composed of rhizospheric soil (cultivated with ) and non-rhizosphere soil (uncultivated soil); and four levels of contamination by sulfentrazone (0, 200, 400, and 800 g ha a.i.). The microbiota associated with the rhizosphere of efficiently reduced sulfentrazone residues in the soil, with better performance at the dose of 200 g ha a.i. Using the PCR-DGGE technique allowed the distinction of two profiles of bacteria in the rhizospheric activity of . The second bacterial profile formed was more efficient in decontaminating soil contaminated with sulfentrazone residue. The microbiota associated with the rhizosphere of has an efficient profile in decontaminating soils with residues equivalent to 200 g ha a.i. the herbicide sulfentrazone.
本研究的目的是利用量化方法（CO2 释放、微生物生物量碳和代谢商）和细菌鉴定（PCR-DGGE 技术）来确定微生物根际在利用苣草修复噻吩磺隆中的效率。该实验采用完全随机设计，2x4 析因设计，4 次重复。处理由根际土壤（用苣草栽培）和非根际土壤（未栽培土壤）组成；以及噻吩磺隆污染的四个水平（0、200、400 和 800 g ha a.i.）。与苣草根际相关的微生物群有效地降低了土壤中的噻吩磺隆残留，在 200 g ha a.i. 的剂量下表现更好。使用 PCR-DGGE 技术可以区分苣草根际活性中两种细菌的特征。形成的第二种细菌特征在去除污染有噻吩磺隆残留的土壤方面更有效。与苣草根际相关的微生物群在去除相当于 200 g ha a.i. 的噻吩磺隆残留的土壤方面具有有效的特征。
