Karim Md Robiul, Li Junqiang, Harun Anas Bin, Rume Farzana Islam, Zhang Longxian
Department of Medicine, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.
One Health. 2024 Feb 3;18:100692. doi: 10.1016/j.onehlt.2024.100692. eCollection 2024 Jun.
is a gastro-intestinal protozoan parasite that has been found to infect both humans and livestock. This study investigated the parasite in 998 fecal samples from Bangladeshi children ( = 299) and calves ( = 699) to determine its prevalence, genetic variation, and zoonotic importance. The nested PCR and sequencing of the SSU rRNA gene in the samples showed a infection rate of 2.3% (7/299) in children and 15.7% (110/699) in calves. Statistical analysis revealed insignificant variations in infections among children across age, gender, and study area, while in calves, the infection rate significantly differed based on location and breed. Genotyping of seven human isolates of confirmed ( = 5) and ( = 2). After characterizing 110 isolates from calves, ( = 55), ( = 29), ( = 14), ( = 10), (n = 1), and (n = 1) were identified. and -positive samples were further subjected to nested PCR and sequencing of the glycoprotein 60 () gene for subtyping. Four subtypes (IaA19R3, IaA23R3, IbA9G3, and IdA15G1) and one subtype (IIdA15G1) were observed. In conclusion, was prevalent in calves but less common in children in the study locations, and the presence of zoonotic species and subtypes in calves raises concerns regarding zoonotic transmission to humans.
是一种胃肠道原生动物寄生虫，已发现可感染人类和家畜。本研究调查了来自孟加拉国儿童（n = 299）和小牛（n = 699）的998份粪便样本中的这种寄生虫，以确定其流行率、遗传变异和人畜共患病重要性。对样本中SSU rRNA基因进行巢式PCR和测序显示，儿童感染率为2.3%（7/299），小牛感染率为15.7%（110/699）。统计分析显示，不同年龄、性别和研究区域的儿童感染情况差异不显著，而在小牛中，感染率因地点和品种而异。对7株人类分离株进行基因分型，确定为E. histolytica（n = 5）和E. dispar（n = 2）。对来自小牛的110株E. histolytica分离株进行鉴定后，确定为E. histolytica（n = 55）、E. dispar（n = 29）、E. moshkovskii（n = 14）、E. bangladeshi（n = 10）、Entamoeba sp. 1（n = 1）和Entamoeba sp. 2（n = 1）。对E. histolytica和E. dispar阳性样本进一步进行巢式PCR和糖蛋白60（gp60）基因测序以进行亚型分析。观察到4种E. histolytica亚型（IaA19R3、IaA23R3、IbA9G3和IdA15G1）和1种E. dispar亚型（IIdA15G1）。总之，在研究地点，E. histolytica在小牛中普遍存在，但在儿童中较少见，小牛中存在人畜共患的E. histolytica物种和亚型引发了对人畜共患传播给人类的担忧。
