Chaudhry Shewta, Kojom Foko Loick Pradel, Narang Geetika, Hawadak Joseph, Arya Aditi, Pande Veena, Singh Vineeta
Cell Biology Laboratory and Malaria Parasite Bank, ICMR-National Institute of Malaria Research, New Delhi, India.
Department of Biotechnology, Kumaun University, Nainital, Uttarakhand 263001 India.
Indian J Microbiol. 2024 Jun;64(2):583-592. doi: 10.1007/s12088-024-01200-1. Epub 2024 Feb 6.
This study analysed the genetic diversity of DBL1α domain of gene in severe and non-severe malaria patients from Delhi and Mewat in Northern India. After confirming infection, samples were cloned and the gene DBL1α domain was sequenced. Out of 377 cloned DBL sequences, 194 were from severe samples and 183 from non-severe samples. Proportion of DBL1α sequences belonging to groups 1, 4 and 5 were significantly higher in severe isolates as compared to non-severe isolates-group 1 (4.1% vs 1.09%, = 0.0333), group 4 (69.58% vs 74.31%, < 0.0001), and group 5 (19.58% vs 10.38%, < 0.0001). Conversely, higher proportion of group 2 was observed in non-severe isolates (0% vs 3.82%, = 0.0350). Highest diversity was seen in PoLV4 motif of severe and non-severe isolates and like other DBL1α sequences reported from several geographical areas (Africa, Americas, Asia, and Oceania). A total of 247 DBL1α domain haplotypes were found in this study where 139 (56.27%) haplotypes are novel and not reported from India till date. These findings could aid in developing effective malaria interventions, including vaccine and drug targets, by understanding the existing antigenic diversity and vulnerabilities in the parasite's genetic makeup.
The online version contains supplementary material available at 10.1007/s12088-024-01200-1.
本研究分析了印度北部德里和默瓦特地区重症和非重症疟疾患者中该基因DBL1α结构域的遗传多样性。确认感染后，对样本进行克隆并对该基因的DBL1α结构域进行测序。在377个克隆的DBL序列中，194个来自重症样本，183个来自非重症样本。与非重症分离株相比，重症分离株中属于第1、4和5组的DBL1α序列比例显著更高——第1组（4.1%对1.09%，P = 0.0333），第4组（69.58%对74.31%，P < 0.0001），第5组（19.58%对10.38%，P < 0.0001）。相反，在非重症分离株中观察到第2组的比例更高（0%对3.82%，P = 0.0350）。在重症和非重症分离株的PoLV4基序中观察到最高的多样性，与几个地理区域（非洲、美洲、亚洲和大洋洲）报道的其他DBL1α序列一样。本研究共发现247个DBL1α结构域单倍型，其中139个（56.27%）单倍型是新的，迄今为止印度尚未报道。这些发现有助于通过了解寄生虫基因构成中现有的抗原多样性和脆弱性来开发有效的疟疾干预措施，包括疫苗和药物靶点。
在线版本包含可在10.1007/s12088 - 024 - 01200 - 1获取的补充材料。
