Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China.
J Antimicrob Chemother. 2024 Sep 3;79(9):2246-2250. doi: 10.1093/jac/dkae220.
To establish the epidemiology cut-off (ECOFF) values of eravacycline against Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter baumannii and Staphylococcus aureus, from a multi-centre study in China.
We collected 2500 clinical isolates from five hospitals in China from 2017 to 2020. The MICs of eravacycline were determined using broth microdilution. The ECOFF values of eravacycline against the five species commonly causing cIAIs were calculated using visual estimation and ECOFFinder following the EUCAST guideline.
The MICs of eravacycline against all the strains were in the range of 0.004-16 mg/L. The ECOFF values of eravacycline were 0.5 mg/L for E. coli, 2 mg/L for K. pneumonia and E. cloacae, and 0.25 mg/L for A. baumannii and S. aureus, consistent with the newest EUCAST publication of eravacycline ECOFF values for the populations. No discrepancy was found between the visually estimated and 99.00% ECOFF values calculated using ECOFFinder.
The determined ECOFF values of eravacycline against the five species can assist in distinguishing wild-type from non-wild-type strains. Given its promising activity, eravacycline may represent a member of the tetracycline class in treating cIAIs caused by commonly encountered Gram-negative and Gram-positive pathogens.
建立依拉环素对大肠埃希菌、肺炎克雷伯菌、阴沟肠杆菌、鲍曼不动杆菌和金黄色葡萄球菌的流行病学折点（ECOFF）值，该研究来自中国的一项多中心研究。
我们收集了 2017 年至 2020 年来自中国五家医院的 2500 株临床分离株。采用肉汤微量稀释法测定依拉环素的 MIC。根据欧盟药敏委员会（EUCAST）指南，采用目测法和 ECOFFinder 计算依拉环素对引起 cIAIs 的五种常见病原体的 ECOFF 值。
依拉环素对所有菌株的 MIC 范围为 0.004-16mg/L。依拉环素对大肠埃希菌、肺炎克雷伯菌和阴沟肠杆菌的 ECOFF 值为 0.5mg/L，对鲍曼不动杆菌和金黄色葡萄球菌的 ECOFF 值为 0.25mg/L，与依拉环素 ECOFF 值的最新 EUCAST 出版物一致。目测法和 ECOFFinder 计算的 99.00% ECOFF 值之间没有差异。
确定的依拉环素对这五种病原体的 ECOFF 值有助于区分野生型和非野生型菌株。鉴于其有前景的活性，依拉环素可能成为治疗常见革兰氏阴性和革兰氏阳性病原体引起的 cIAIs 的四环素类药物的一员。
