Institute of Biological Science and Technology, Guangxi Academy of Sciences, Nanning, Guangxi, China.
College of Life Science and Technology, Guangxi University, Nanning, Guangxi, China.
mSphere. 2024 Aug 28;9(8):e0042824. doi: 10.1128/msphere.00428-24. Epub 2024 Jul 16.
f. sp. () poses a significant threat to banana crops as a lethal fungal pathogen. The global spread of underscores the formidable challenges associated with traditional management methods in combating this pathogen. This study delves into the hypovirulence-associated mycovirus in . From strain LA6, we isolated and characterized a novel member of the family, named Hadaka virus 1 strain LA6 (HadV1-LA6). HadV1-LA6 comprises 10 genomic RNA segments, with RNA1 to RNA7 sharing 80.9%-95.0% amino acid sequence identity with known HadV1-7n, while RNA8 to RNA10 display significantly lower identity. HadV1-LA6 demonstrates horizontal transmission capabilities in an all-or-none fashion between different strains via coculturing. Phenotypic comparisons highlight that HadV1-LA6 significantly reduces the growth rates of its host fungus under cell wall stress and oxidative stress conditions. Importantly, HadV1-LA6 attenuates 's virulence in detached leaves and banana plants. This study represents the first introduction of a novel hypovirulence-associated Hadaka virus 1 in .IMPORTANCEFusarium wilt of banana (FWB) is a severe fungal disease caused by soil-borne f. sp. (). Among various strategies, biocontrol emerges as a safe, ecologically friendly, and cost-effective approach to managing FWB. In this study, we focus on exploring the potential of a novel hypovirulent member of hadakavirid, HadV1-LA6. Previous reports suggest that HadV1 shows no apparent effect on the host. However, through phenotypic assessments, we demonstrate that HadV1-LA6 significantly impedes the growth rates of its host fungus under stress conditions. More importantly, HadV1-LA6 exhibits a remarkable capacity to attenuate 's virulence in detached leaves and banana plants. Furthermore, HadV1-LA6 could be horizontally transmitted between different strains, presenting a promising resource for revealing the molecular mechanism of the interaction between Hadaka virus 1 and its host.
f. sp. （）是香蕉作物的一种致命真菌病原体，构成了重大威胁。 在全球范围内传播，突显出传统管理方法在应对这种病原体方面所面临的巨大挑战。本研究深入探讨了 中的弱毒相关真菌病毒。我们从 LA6 菌株中分离并鉴定了一种新的 科成员，命名为 Hadaka 病毒 1 株 LA6（HadV1-LA6）。HadV1-LA6 由 10 个基因组 RNA 片段组成，RNA1 到 RNA7 与已知的 HadV1-7n 具有 80.9%-95.0%的氨基酸序列同一性，而 RNA8 到 RNA10 则显示出明显较低的同一性。HadV1-LA6 通过共培养以全有或全无的方式在不同 菌株之间进行水平传播。表型比较表明，HadV1-LA6 在细胞壁应激和氧化应激条件下显著降低其宿主真菌的生长速率。重要的是，HadV1-LA6 减弱了 在离体叶片和香蕉植株中的毒力。本研究首次在 中引入了一种新的弱毒相关 Hadaka 病毒 1。
香蕉枯萎病（FWB）是由土壤传播的 f. sp. （）引起的一种严重真菌病。在各种策略中，生物防治作为一种安全、生态友好和具有成本效益的方法来管理 FWB 脱颖而出。在本研究中，我们专注于探索新型弱毒 Hadakavirid 成员 HadV1-LA6 的潜力。以前的报告表明，HadV1 对宿主没有明显的影响。然而，通过表型评估，我们表明 HadV1-LA6 在应激条件下显著抑制其宿主真菌的生长速率。更重要的是，HadV1-LA6 在离体叶片和香蕉植株中表现出显著的减毒能力。此外，HadV1-LA6 可以在不同的 菌株之间水平传播，为揭示 Hadaka 病毒 1 与其宿主之间的相互作用的分子机制提供了有前途的资源。
