Ahmed Mohammed Yahya, Gorish Babbiker Mohammed Taher, Alhaj Esraa Mohammed, Elrhim Mayasir Abd Elmoniem Abd, Siddig Shimaa Saifaldeen, Altayb Hisham N
Department of Microbiology, Faculty of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan.
Department of Microbiology, Faculty of Medical Laboratory Science, Omdurman Islamic University, Omdurman, Sudan.
J Med Case Rep. 2024 Jul 17;18(1):326. doi: 10.1186/s13256-024-04663-4.
Escherichia coli is a major human pathogen responsible for a broad range of clinical illnesses. It has been linked to endemic and epidemic nosocomial diseases caused by multidrug-resistant pathogens in Sudan as well as throughout the globe.
A 76-year-old African woman arrived at Saad Rashwan Medical Centre complaining of backaches and discomfort during urination. Throughout the preceding 5 years, the patient had recurrent urinary tract infections. Following overnight incubation at 37 °C, Escherichia coli was found in her midstream urine specimen on cysteine lactose electrolyte deficient agar media. Minimum inhibitory concentration (colorimetric/turbidimetric method) was employed to test a wide range of antimicrobial drugs against this bacterial strain, and the results revealed significant multidrug resistance. QIAamp DNA Mini Kit was used to obtain DNA Template from the purified Escherichia coli (Qiagen, Hilden, Germany). The bacterial whole-genome sequence was done by Novogene company (Hong Kong) using Illumina HiSeq 2500 (Illumina, San Diego, CA, USA), followed by whole genome reconstructions, and identification of antibiotic-resistant genes. Phylogenetic analysis revealed that our strain was related to the Escherichia coli DSM30083 ( genome sequence ID: CP033092.2) from the USA. Our strain possessed the following antimicrobial-resistant genes: aminoglycoside (kdpE, baeR, cpxA, aadA5), nitroimidazole (msbA), phosphonic acid (mdtG), tetracycline (emrY), macrolide, penam, tetracycline, (evgA, TolC, H-NS), fluoroquinolone, cephalosporin, glycylcycline, penam, tetracycline, rifamycin, phenicol antibiotic, disinfecting agents and antiseptics (acrB; marA), sulfonamide (sul1), macrolide (Mrx), cephalosporin, penam (CTX-M-15), carbapenem, cephalosporin, and penam (OXA-1).
This study found that the isolated Escherichia coli strain had varied antimicrobial resistance genes on the basis of whole-genome sequencing and phenotypic resistance analyses. Whole-genome sequencing is critical for control and preventative methods to battle the growing threat of antimicrobial resistance. A larger investigation is recommended for improved generalization of results.
大肠杆菌是导致多种临床疾病的主要人类病原体。在苏丹以及全球范围内，它都与由多重耐药病原体引起的地方性和流行性医院感染疾病有关。
一名76岁的非洲女性来到萨阿德·拉什万医疗中心，主诉背痛和排尿不适。在过去的5年里，该患者反复发生尿路感染。在37°C过夜培养后，在半胱氨酸乳糖电解质缺乏琼脂培养基上的中段尿标本中发现了大肠杆菌。采用最低抑菌浓度（比色法/比浊法）对该菌株进行多种抗菌药物测试，结果显示出显著的多重耐药性。使用QIAamp DNA Mini试剂盒从纯化的大肠杆菌中获取DNA模板（德国希尔德的Qiagen公司）。细菌全基因组测序由香港的诺禾致源公司使用Illumina HiSeq 2500（美国加利福尼亚州圣地亚哥的Illumina公司）完成，随后进行全基因组重建，并鉴定抗生素耐药基因。系统发育分析表明，我们的菌株与来自美国的大肠杆菌DSM30083（基因组序列ID：CP033092.2）相关。我们的菌株拥有以下抗菌耐药基因：氨基糖苷类（kdpE、baeR、cpxA、aadA5）、硝基咪唑类（msbA）、膦酸类（mdtG）、四环素类（emrY）、大环内酯类、青霉烯类、四环素类（evgA、TolC、H-NS）、氟喹诺酮类、头孢菌素类、甘氨酰环素类、青霉烯类、四环素类、利福霉素类、氯霉素类抗生素、消毒剂和防腐剂（acrB；marA）、磺胺类（sul1）、大环内酯类（Mrx）、头孢菌素类、青霉烯类（CTX-M-15）、碳青霉烯类、头孢菌素类和青霉烯类（OXA-1）。
本研究发现，基于全基因组测序和表型耐药性分析，分离出的大肠杆菌菌株具有多种抗菌耐药基因。全基因组测序对于对抗日益增长的抗菌耐药性威胁的控制和预防方法至关重要。建议进行更大规模的调查以更好地推广研究结果。
