Schulze Friederike, Määttä Juhani, Grad Sybille, Heggli Irina, Brunner Florian, Farshad Mazda, Distler Oliver, Karppinen Jaro, Lotz Jeffrey, Dudli Stefan
Center of Experimental Rheumatology, Department of Rheumatology University Hospital Zurich, University of Zurich Zurich Switzerland.
Department of Physical Medicine and Rheumatology Balgrist University Hospital, Balgrist Campus, University of Zurich Zurich Switzerland.
JOR Spine. 2024 Jul 15;7(3):e1337. doi: 10.1002/jsp2.1337. eCollection 2024 Sep.
Modic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, and MC3. The inter-observer variability of the MRI diagnosis is high, therefore a diagnostic serum biomarker complementing the MRI to facilitate diagnosis and follow-up would be of great value.
We used a highly sensitive and reproducible proteomics approach: DIA/SWATH-MS to find serum biomarkers in a subset of the Northern Finland Birth Cohort 1966. Separately, we measured a panel of factors involved in inflammation and angiogenesis to confirm some potential biomarkers published before with an ELISA-based method called V-Plex.
We found neither an association between the serum concentrations of the proteins detected with DIA/SWATH-MS with the presence of MC, nor a correlation with the size of the MC lesions. We did not find any association between the factors measured with the V-Plex and the presence of MC or their size.
Altogether, our study suggests that a robust and generally usable biomarker to facilitate the diagnosis of MC cannot readily be found in serum.
Modic改变（MC）是椎骨的骨髓病变，可通过磁共振成像（MRI）在退变椎间盘附近检测到。根据其在T1加权和T2加权图像上的表现定义，有三种可相互转换的类型：MC1、MC2和MC3。MRI诊断的观察者间变异性很高，因此一种补充MRI以促进诊断和随访的诊断血清生物标志物将具有很大价值。
我们使用了一种高度灵敏且可重复的蛋白质组学方法：数据独立采集/质谱（DIA/SWATH-MS），在1966年芬兰北部出生队列的一个子集中寻找血清生物标志物。另外，我们测量了一组参与炎症和血管生成的因子，以用一种基于酶联免疫吸附测定（ELISA）的方法V-Plex确认之前发表的一些潜在生物标志物。
我们既未发现用DIA/SWATH-MS检测到的蛋白质的血清浓度与MC的存在之间存在关联，也未发现与MC病变大小存在相关性。我们未发现用V-Plex测量的因子与MC的存在或其大小之间存在任何关联。
总体而言，我们的研究表明，在血清中不容易找到一种可靠且普遍可用的促进MC诊断的生物标志物。
