Zhang Ling-Li, Du Ming-Yan, Du Xin, Duan Jie, Yao Dong-Mei, Jing Jing, Feng Chun, Song Lin
Department of Gynaecology, Maternal and Child Health Hospital of Hubei Province, Wuhan 430075, Hubei Province, China.
Department of Gynaecology, China Resources WISCO General Hospital, Wuhan 430080, Hubei Province, China.
World J Clin Cases. 2024 Jul 16;12(20):4146-4153. doi: 10.12998/wjcc.v12.i20.4146.
Cervical intraepithelial neoplasia (CIN) is an important precursor of cervical cancer. Early detection and treatment can reduce the incidence of cervical cancer.
To investigate the detection rate of human papillomavirus (HPV) E6/E7 mRNA in cervical tissue of patients with different types of epithelial cell neoplasia (CIN) and its relationship with CIN progression and diagnosis.
One hundred women with HPV infection detected by cervical exfoliation cytology between January 2022 and January 2023 were retrospectively selected. These patients were graded CIN based on colposcopy and cervical pathology. The positive expression rates of HPV E6/E7 mRNA and HPV [polymerase chain reaction (PCR)-reverse dot crossing] were compared among all groups. Patients with HPV E6/E7 mRNA expression in the grade 1 CIN group were followed up for 1 yr. The relationship between atypical squamous epithelium and high malignant epithelial neoplasia was investigated by univariate and multivariate analysis.
The diagnostic sensitivity, specificity, and sensitivity of PCR-reverse point hybridization technology for secondary CIN were 70.41%, 70.66%, and 0.714, respectively. Sensitivity and specificity for secondary CIN were 752% and 7853%, respectively, the area under the curve value was 0.789. Logistic Multifactorial model analysis revealed that the HPV positive rates and the HPV E6/E7 mRNA positive rates were independent risk factors of CIN grade I ( < 0.05). In CIN grade I patients with positive for HPV E6/E7 mRNA, in its orientation to grade CIN patients, in its orientation to grade CIN patients, at 69.2%, compared with patients negative for HPV E6/E7 mRNA (30.8%), significant difference ( < 0.05).
HPV E6/E7 mRNA and HPV (PCR-reverse dot hybrid) positive expression have a close relationship with CIN-grade disease progression and is an independent risk factor for high-grade CIN lesions.
宫颈上皮内瘤变（CIN）是宫颈癌的重要前驱病变。早期检测和治疗可降低宫颈癌的发病率。
探讨不同类型上皮细胞内瘤变（CIN）患者宫颈组织中人乳头瘤病毒（HPV）E6/E7 mRNA的检出率及其与CIN进展和诊断的关系。
回顾性选取2022年1月至2023年1月间经宫颈脱落细胞学检查确诊为HPV感染的100例女性患者。根据阴道镜检查及宫颈病理结果对这些患者进行CIN分级。比较各组HPV E6/E7 mRNA和HPV[聚合酶链反应（PCR）-反向点杂交]的阳性表达率。对1级CIN组中HPV E6/E7 mRNA表达阳性的患者进行1年随访。采用单因素和多因素分析研究非典型鳞状上皮与高恶性上皮内瘤变之间的关系。
PCR-反向点杂交技术对二级CIN的诊断敏感性、特异性和符合率分别为70.41%、70.66%和0.714。对二级CIN的敏感性和特异性分别为75.2%和78.53%，曲线下面积值为0.789。Logistic多因素模型分析显示，HPV阳性率和HPV E6/E7 mRNA阳性率是CIN 1级的独立危险因素（P<0.05）。在HPV E6/E7 mRNA阳性的CIN 1级患者中，其发展为CIN患者的比例为69.2%，与HPV E6/E7 mRNA阴性患者（30.8%）相比，差异有统计学意义（P<0.05）。
HPV E6/E7 mRNA和HPV（PCR-反向点杂交）阳性表达与CIN疾病进展密切相关，是高级别CIN病变的独立危险因素。
