de Carvalho Rocha Koga Rosemary, Custodio de Souza Gisele, Tavares de Lima Teixeira Abrahão Victor, Ferreira Adriana Maciel, Sánchez-Ortiz Brenda Lorena, Silva Abreu Lucas, Fechine Tavares Josean, Carvalho José Carlos Tavares
Program in Pharmaceutical Innovation, Department of Biological and Health Sciences, Universidade Federal do Amapá, Macapá, Amapá, Brazil.
Research Laboratory of Drugs, Department of Biological and Health Sciences, Universidade Federal do Amapá, Macapá, Amapá, Brazil.
Pharm Biol. 2024 Dec;62(1):577-591. doi: 10.1080/13880209.2024.2374806. Epub 2024 Jul 17.
The botanical species Aublet (Leguminosae-Cercidoideae) is traditionally used in the Amazon for medicinal purposes.
The acute toxicity of the hydroethanolic extracts from leaves and stems (HELBg and HESBg) was evaluated in zebrafish (), with emphasis on the embryonic developmental stage and adult alterations.
Extracts were analyzed on LC-DAD-MS/MS. Zebrafish eggs were inoculated individually with concentrations of HELBg and HESBg (0.25, 0.5, 0.75, 1.0, and 1.5 µg/mL), observed for 96 h. Adult zebrafish were treated with a single oral dose (100, 200, 500, 1000, and 2000 mg/kg) of HELBg and HESBg, observed for 48 h.
HELBg and HESBg analysis detected 55 compounds. Both extracts exhibited toxicity, including embryo coagulation at higher doses of HELBg and absence of heartbeats in embryos at all doses of HESBg. Behavioral variations were observed; tissue alterations in adult zebrafish were found at the highest doses, primarily in the liver, intestine, and kidneys because of HELBg and HESBg effects. The LD of HESBg was 1717 mg/kg, while HELBg exceeded the limit dose of 2000 mg/kg.
The study on acute toxicity of extracts exhibits significant toxic potential, emphasizing effects on embryonic and adult zebrafish. The results suggest relative safety of the species preparations, encouraging further clinical trials on potential biological activities.
植物物种奥布莱特（豆科 - 紫荆亚科）在亚马逊地区传统上用于药用。
评估叶片和茎部的水乙醇提取物（HELBg和HESBg）对斑马鱼的急性毒性，重点关注胚胎发育阶段和成年鱼的变化。
提取物通过LC - DAD - MS/MS进行分析。将斑马鱼卵分别接种不同浓度的HELBg和HESBg（0.25、0.5、0.75、1.0和1.5μg/mL），观察96小时。成年斑马鱼经口单次给予HELBg和HESBg（100、200、500、1000和2000mg/kg），观察48小时。
对HELBg和HESBg的分析检测到55种化合物。两种提取物均表现出毒性，包括高剂量HELBg导致胚胎凝固，所有剂量的HESBg均使胚胎无心跳。观察到行为变化；在最高剂量下发现成年斑马鱼有组织改变，主要是由于HELBg和HESBg的作用导致肝脏、肠道和肾脏出现病变。HESBg的LD50为1717mg/kg，而HELBg超过了2000mg/kg的极限剂量。
提取物的急性毒性研究显示出显著的毒性潜力，突出了对胚胎和成年斑马鱼的影响。结果表明该物种制剂具有相对安全性，鼓励对潜在生物活性进行进一步的临床试验。
