Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
Biotechnology School, Jiangnan University,Wuxi 214122,China.
Yi Chuan. 2024 Jul;46(7):552-559. doi: 10.16288/j.yczz.24-038.
During meiosis, defects in cohesin localization within the centromere region can result in various diseases. Accurate cohesin localization depends on the Mis4-Ssl3 loading complex. Although it is known that cohesin completes the loading process with the help of the loading complex, the mechanisms underlying its localization in the centromere region remain unclear. Previous studies suggest cohesin localization in the centromere is mediated by phosphorylation of centromeric proteins. In this study, we focused on the Fta2 protein, a component of the Sim4 centromere protein complex. Using bioinformatics methods, potential phosphorylation sites were identified, and and mutants were constructed in . The phenotypes of these mutants were characterized through testing thiabendazole (TBZ) sensitivity and fluorescent microscopy localization. Results indicated that Fta2 phosphorylation did not impact mitosis but affected chromosome segregation during meiosis. This study suggests that Fta2 phosphorylation is vital for meiosis and may be related to the specific localization of cohesin during this process.
在减数分裂过程中，如果着丝粒区域内的黏连蛋白定位出现缺陷，可能会导致各种疾病。黏连蛋白的准确定位依赖于 Mis4-Ssl3 加载复合物。虽然已知黏连蛋白在加载复合物的帮助下完成加载过程，但它在着丝粒区域的定位机制尚不清楚。先前的研究表明，着丝粒处的黏连蛋白定位是通过着丝粒蛋白的磷酸化介导的。在这项研究中，我们专注于 Fta2 蛋白，它是 Sim4 着丝粒蛋白复合物的一个组成部分。利用生物信息学方法，鉴定了潜在的磷酸化位点，并在 中构建了 和 突变体。通过噻菌灵（TBZ）敏感性测试和荧光显微镜定位，对这些突变体的表型进行了表征。结果表明，Fta2 磷酸化并不影响有丝分裂，但会影响减数分裂过程中的染色体分离。这项研究表明，Fta2 磷酸化对减数分裂至关重要，可能与黏连蛋白在该过程中的特定定位有关。
