Science Center for Future Foods, Jiangnan University, Wuxi 214122, China.
School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Yi Chuan. 2024 Aug;46(8):649-660. doi: 10.16288/j.yczz.24-035.
The localization of the meiotic specific regulatory molecule Moa1 to the centromere is regulated by the kinetochore protein CENP-C, and participates in the cohesion of sister chromatids in the centromere region mediated by the cohesin Rec8. To examine the interaction of these proteins, we analyzed the interactions between Moa1 and Rec8, CENP-C by yeast two-hybrid assays and identified several amino acid residues in Moa1 required for the interaction with CENP-C and Rec8. The results revealed that the interaction between Moa1 and CENP-C is crucial for the Moa1 to participate in the regulation of monopolar attachment of sister kinetochores. However, mutation at S143 and T150 of Moa1, which are required for interaction with Rec8 in the two-hybrid assay, did not show significant defects. Mutations in amino acid residues may not be sufficient to interfere with the interaction between Moa1 and Rec8 . Further research is needed to determine the interaction domain between Moa1 and Rec8. This study revealed specific amino acid sites at which Moa1 affects the meiotic homologous chromosome segregation, providing a deeper understanding of the mechanism of meiotic chromosome segregation.
减数分裂特异性调节分子 Moa1 定位于着丝粒的过程受动粒蛋白 CENP-C 调控，并通过着丝粒区域的黏合蛋白 Rec8 参与姐妹染色单体的黏合。为了研究这些蛋白质的相互作用，我们通过酵母双杂交实验分析了 Moa1 与 Rec8、CENP-C 之间的相互作用，并确定了 Moa1 与 CENP-C 和 Rec8 相互作用所需的几个氨基酸残基。结果表明，Moa1 与 CENP-C 的相互作用对于 Moa1 参与调节姐妹动粒的单极附着至关重要。然而，在双杂交实验中与 Rec8 相互作用所需的 Moa1 的 S143 和 T150 突变并没有显示出明显的缺陷。氨基酸残基的突变可能不足以干扰 Moa1 与 Rec8 之间的相互作用。需要进一步研究以确定 Moa1 与 Rec8 之间的相互作用域。本研究揭示了 Moa1 影响减数分裂同源染色体分离的特定氨基酸位点，为减数分裂染色体分离的机制提供了更深入的了解。
