Department of Hepatobiliary Pancreatogastric Surgery, Shanxi Province Cancer Hospital/Shanxi Hospital Affiliated to Caner Hospital Chinese Academy of Medical Science/Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, 030013, China.
Department of Gastroenterology, Jilin Province FAW General Hospital, Changchun, 130013, China.
J Environ Pathol Toxicol Oncol. 2024;43(4):43-52. doi: 10.1615/JEnvironPatholToxicolOncol.2024053208.
Gastric cancer, a prevalent malady within the digestive tract, has a complex pathological mechanism and numerous patients. The regulation of gastric cancer process by long non-coding RNA (lncRNA) presented new prospects for the study of its molecular mechanism and the treatment of patients. The abnormal expressed genes in gastric cancer were screened by GSE193109 dataset. The correlation between LINC01278 and the likelihood of survival in patients suffering from gastric cancer was investigated by Kaplan-Meier survival curve and multivariate Cox analysis. LINC01278 in gastric cancer tissue samples and cells was verified via RT-qPCR. The cell counting kit-8 (CCK-8) and transwell assay were selected to detect the growth activity of gastric cancer cells. The association between LINC01278 and miR-129-5p was validated through luciferase reporter assay and RNA-binding protein immunoprecipitation (RIP) assay. Correlation analysis of clinical features revealed an association between LINC01278 and the prognosis in gastric cancer patients. LINC01278 was actively expressed in gastric cancer, which exerts a tumor-promoting effect. Silencing LINC01278 suppressed the biological function of tumor cells through spongiform miR-129-5p. LINC01278 has the potential to serve as a novel biomarker, offering new avenues of research for the prognosis and treatment of gastric cancer.
胃癌是一种常见的消化道恶性肿瘤，其发病机制复杂，患者众多。长链非编码 RNA（lncRNA）调控胃癌进程为其分子机制研究和患者治疗提供了新的前景。通过 GSE193109 数据集筛选胃癌中异常表达的基因。通过 Kaplan-Meier 生存曲线和多因素 Cox 分析研究 LINC01278 与胃癌患者生存概率的相关性。通过 RT-qPCR 验证胃癌组织样本和细胞中的 LINC01278。选择细胞计数试剂盒-8（CCK-8）和 Transwell 检测法检测胃癌细胞的生长活性。通过荧光素酶报告基因检测和 RNA 结合蛋白免疫沉淀（RIP）检测验证 LINC01278 与 miR-129-5p 的关联。临床特征相关性分析显示 LINC01278 与胃癌患者的预后相关。LINC01278 在胃癌中呈高表达，发挥促瘤作用。沉默 LINC01278 通过海绵状 miR-129-5p 抑制肿瘤细胞的生物学功能。LINC01278 有望成为一种新的生物标志物，为胃癌的预后和治疗提供新的研究途径。
