Master Program of Biomedical Science Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia; Department of Biomedical Science, Faculty of Medicine, Parahyangan Catholic University, Jalan Ciumbuleuit no.94, Bandung, 40141, Indonesia.
Department of Histology and Cell Biology Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia.
Placenta. 2024 Sep 2;154:162-167. doi: 10.1016/j.placenta.2024.07.006. Epub 2024 Jul 14.
Poor placental angiogenesis is associated with several pregnancy complications including fetal growth restriction (FGR), which causes low birth weight (LBW) babies to have a high risk of growth disorders and metabolic disorders in adulthood. Recent research using syncytin knock-out mice showed significant disruption in the growth of placental vascularization. Syncytin-1 which encoded by ERVW-1 gene, is proposed to have a role in placental angiogenesis, but its relationship with other proangiogenic factors such as vascular endothelial growth factor (VEGF) in the placenta of LBW babies has not yet been determined. By knowing the mechanisms of FGR, more proactive preventive and therapeutic measures can be taken in the future. This study aimed to determine the expression of ERVW-1, proangiogenic gene VEGF and its receptor (FLT-1), and hypoxia inducible factor-1 (HIF-1) in LBW placentas, and investigate the relationship between these genes' expression in the placenta of LBW babies.
Total RNA was extracted from placental tissue. Total RNA is used as a cDNA synthesis template, followed by qRT-PCR. Correlations of ERVW-1, VEGF, FLT-1 and HIF-1 genes' expression were analyzed by linear regression.
The age and body mass index of mothers with LBW and normal birth weight (NBW) babies were not significantly different. ERVW-1 expression in LBW placentas was lower than in NBW placentas, but VEGF, FLT-1 and HIF-1 expressions were higher. ERVW-1 was negatively correlated with HIF-1 and VEGF.
Low expression of ERVW-1 in the placenta of LBW babies may result in impaired placental angiogenesis and possibly lead to hypoxia.
不良的胎盘血管生成与多种妊娠并发症有关，包括胎儿生长受限（FGR），这会导致低出生体重（LBW）婴儿在成年后患生长障碍和代谢紊乱的风险增加。最近使用合胞体蛋白敲除小鼠的研究表明，胎盘血管生成的生长受到显著破坏。由 ERVW-1 基因编码的合胞体蛋白 1（Syncytin-1）被认为在胎盘血管生成中具有作用，但它与胎盘内其他促血管生成因子（如血管内皮生长因子（VEGF））的关系尚未确定。通过了解 FGR 的机制，可以在未来采取更积极的预防和治疗措施。本研究旨在确定 LBW 胎盘中 ERVW-1、促血管生成基因 VEGF 及其受体（FLT-1）和缺氧诱导因子-1（HIF-1）的表达，并探讨这些基因在 LBW 婴儿胎盘中的表达之间的关系。
从胎盘组织中提取总 RNA。总 RNA 用作 cDNA 合成模板，然后进行 qRT-PCR。通过线性回归分析 ERVW-1、VEGF、FLT-1 和 HIF-1 基因表达的相关性。
LBW 和正常出生体重（NBW）婴儿的母亲年龄和体重指数无显著差异。LBW 胎盘 ERVW-1 的表达低于 NBW 胎盘，但 VEGF、FLT-1 和 HIF-1 的表达较高。ERVW-1 与 HIF-1 和 VEGF 呈负相关。
LBW 婴儿胎盘 ERVW-1 的低表达可能导致胎盘血管生成受损，并可能导致缺氧。
