Department of General Pathology/Laboratory of Comparative Pathology, Federal University of Minas Gerais, Av. Pres. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, 31270-091, Brazil; Department of Veterinary Pathology, Universidade Vila Velha, Vila Velha, Espirito Santo, 29101-420, Brazil.
Department of Surgery, School of Medicine, Federal University of Minas Gerais, Av. Pres. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, 31270-091, Brazil.
J Comp Pathol. 2024 Aug;213:1-9. doi: 10.1016/j.jcpa.2024.06.004. Epub 2024 Jul 16.
Morphological and immunohistochemical studies of solid arrangement canine mammary carcinomas have shown that the different histological types may be characterized by proliferation of epithelial and/or myoepithelial cells. However, little is known about immunophenotypes and the importance of inflammation as prognostic factors in these neoplasms. The objective of the present study was to characterize the immunophenotype and degree of inflammation in the solid type of canine mammary neoplasm and to investigate their association with metastasis, Ki-67 index, tumour size, necrosis and survival. Sixty-five carcinomas with solid pattern, basaloid carcinomas, solid papillary carcinomas, malignant adenomyoepitheliomas (MAMEs) or malignant myoepitheliomas (MMEs) were investigated. Luminal A, luminal B HER2 negative and HER2 positive, HER2 overexpressed and triple negative immunophenotypes were immunolabelled as were Ki-67 protein and cyclooxygenase-2 (Cox-2). Histological peritumoural and intratumoural inflammatory infiltrates were graded (distribution × intensity) and the presence of necrosis identified. We found a statistical difference between histological types and immunophenotypes, with MME and MAME having a higher occurrence of luminal A, whereas most neoplasms had the luminal B HER-negative immunophenotype. There was no correlation between immunophenotype and degree of peri- and intratumoural inflammation, nodal metastasis, necrosis or tumour size. An increased degree of peri- and intratumoural inflammation was significantly associated with lymph node metastasis, and more severe intratumoural inflammation was associated with the presence of tumour necrosis. Tumour size, Ki-67 index and Cox-2 score were not associated with inflammation in either peri- or intratumoural regions. No difference was observed in survival in relation to immunophenotype or degree of inflammation, but the Cox regression model revealed that nodal metastasis influenced the risk of death.
形态学和免疫组织化学研究表明，实体排列的犬乳腺肿瘤的不同组织学类型可能表现为上皮和/或肌上皮细胞的增殖。然而，关于这些肿瘤的免疫表型和炎症作为预后因素的重要性知之甚少。本研究的目的是描述实体型犬乳腺肿瘤的免疫表型和炎症程度，并探讨其与转移、Ki-67 指数、肿瘤大小、坏死和生存的关系。对 65 例具有实体型、基底细胞样型、实体乳头状型、恶性腺肌上皮瘤（MAME）或恶性肌上皮瘤（MME）的癌进行了研究。免疫标记了腔 A、腔 B HER2 阴性和 HER2 阳性、HER2 过表达和三阴性免疫表型，以及 Ki-67 蛋白和环氧化酶-2（Cox-2）。对肿瘤周围和肿瘤内的炎症浸润进行了分级（分布×强度），并确定了坏死的存在。我们发现组织学类型和免疫表型之间存在统计学差异，MAME 和 MME 更常出现腔 A，而大多数肿瘤具有腔 B HER2 阴性免疫表型。免疫表型与肿瘤周围和肿瘤内炎症程度、淋巴结转移、坏死或肿瘤大小之间无相关性。肿瘤周围和肿瘤内炎症程度的增加与淋巴结转移显著相关，而肿瘤内炎症程度的加重与肿瘤坏死的存在相关。肿瘤大小、Ki-67 指数和 Cox-2 评分与肿瘤周围和肿瘤内区域的炎症均无相关性。免疫表型或炎症程度与生存无差异，但 Cox 回归模型显示淋巴结转移影响死亡风险。
