Flecher Mayra C, Reys Marina P, Balabram Débora, Nakagaki Karen Y R, Cassali Geovanni D
Department of General Pathology, Laboratory of Comparative Pathology, Federal University of Minas Gerais, Belo Horizonte 31270-091, Minas Gerais, Brazil.
Department of Veterinary Medicine, Universidade Vila Velha, Vila Velha 29102-920, Espírito Santo, Brazil.
Animals (Basel). 2025 Jan 20;15(2):287. doi: 10.3390/ani15020287.
In canine mammary neoplasms, greater inflammation is associated with higher histological grade, lymphatic invasion, and metastases. This retrospective study assessed the density of peri- and intratumoral tumor-infiltrating lymphocytes (TILs), tumor-associated neutrophils (TANs), and CD3 and CD79 lymphocytes in canine mammary neoplasms with a solid arrangement, and associated such data with histological types, immunophenotype, prognostic factors, cyclooxygenase-2 (Cox-2) expression and overall and cancer-specific survival. Sixty-one neoplasms with a solid arrangement were classified as malignant myoepitheliomas (6/9.8%), solid papillary carcinomas (8/13.1%), carcinomas with a solid pattern (9/14.8%), basaloid carcinomas (BC) (19/31.1%), and malignant adenomyoepitheliomas (19/31.1%). Intra- and peritumoral TILs, TANs, and TCD3 and BCD79 lymphocytes were counted, and based on the resulting median, the neoplasms were divided into low or high cell infiltration. BCs had the lowest density of intratumoral TILs ( = 0.02), and luminal B neoplasms showed a significantly higher density of intratumoral TCD3 than luminal A cases. Neoplasms with a higher density of peritumoral CD3 and CD79 had significantly greater proliferative activity. High infiltration of intratumoral BCD79 lymphocytes was related to nodal metastasis ( = 0.03). Intratumoral TILs and TCD3 were associated with shorter survival time. Therefore, intratumoral lymphocyte infiltration is possibly an important feature in the progression of cancer and influences the survival in bitches with solid arrangement neoplasms.
在犬乳腺肿瘤中，炎症越严重与组织学分级越高、淋巴管浸润及转移相关。本回顾性研究评估了实体型犬乳腺肿瘤中肿瘤周围及瘤内肿瘤浸润淋巴细胞（TILs）、肿瘤相关中性粒细胞（TANs）以及CD3和CD79淋巴细胞的密度，并将这些数据与组织学类型、免疫表型、预后因素、环氧合酶-2（Cox-2）表达以及总生存期和癌症特异性生存期相关联。61例实体型肿瘤被分类为恶性肌上皮瘤（6/9.8%）、实体乳头状癌（8/13.1%）、实体型癌（9/14.8%）、基底样癌（BC）（19/31.1%）和恶性腺肌上皮瘤（19/31.1%）。对瘤内和瘤周的TILs、TANs以及TCD3和BCD79淋巴细胞进行计数，并根据所得中位数将肿瘤分为低细胞浸润或高细胞浸润。BC的瘤内TILs密度最低（ = 0.02），管腔B型肿瘤的瘤内TCD3密度显著高于管腔A型病例。瘤周CD3和CD79密度较高的肿瘤具有显著更高的增殖活性。瘤内BCD79淋巴细胞的高浸润与淋巴结转移相关（ = 0.03）。瘤内TILs和TCD3与较短的生存时间相关。因此，瘤内淋巴细胞浸润可能是癌症进展中的一个重要特征，并影响实体型肿瘤母犬的生存。
