Kanbar Adnan, Weinert Christoph Hubertus, Kottutz David, Thinh La, Abuslima Eman, Kabil Farida, Hazman Mohamed, Egert Björn, Trierweiler Bernhard, Kulling Sabine Emma, Nick Peter
Molecular Cell Biology, Joseph Kölreuter Institute for Plant Sciences, Karlsruhe Institute of Technology, Fritz-Haber-Weg 4, D-76131 Karlsruhe, Germany.
Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, D-76131 Karlsruhe, Germany.
J Exp Bot. 2024 Sep 27;75(18):5857-5879. doi: 10.1093/jxb/erae263.
Domesticated strawberry is susceptible to sudden frost episodes, limiting the productivity of this cash crop in regions where they are grown during early spring. In contrast, the ancestral woodland strawberry (Fragaria vesca) has successfully colonized many habitats of the Northern Hemisphere. Thus, this species seems to harbour genetic factors promoting cold tolerance. Screening a germplasm established in the frame of the German Gene Bank for Crop Wild Relatives, we identified, among 70 wild accessions, a pair with contrasting cold tolerance. By following the physiological, biochemical, molecular, and metabolic responses of this contrasting pair, we identified the transcription factor Cold Box Factor 4 and the dehydrin Xero2 as molecular markers associated with superior tolerance to cold stress. Overexpression of green fluorescent protein fusions with Xero2 in tobacco BY-2 cells conferred cold tolerance to these recipient cells. A detailed analysis of the metabolome for the two contrasting genotypes allows the definition of metabolic signatures correlated with cold tolerance versus cold stress. This work provides a proof-of-concept for the value of crop wild relatives as genetic resources to identify genetic factors suitable to increase the stress resilience of crop plants.
驯化草莓易受突然霜冻影响，这限制了这种经济作物在早春种植地区的产量。相比之下，野生林地草莓（野草莓）已成功在北半球的许多栖息地繁衍。因此，该物种似乎拥有促进耐寒性的遗传因素。通过筛选德国作物野生近缘种基因库框架下建立的种质资源，我们在70个野生种质中鉴定出一对耐寒性相反的种质。通过追踪这一对种质的生理、生化、分子和代谢反应，我们确定了转录因子冷盒因子4和脱水蛋白Xero2作为与优异耐寒性相关的分子标记。Xero2与绿色荧光蛋白融合物在烟草BY - 2细胞中的过表达赋予了这些受体细胞耐寒性。对这两种耐寒性相反的基因型进行代谢组详细分析，能够确定与耐寒性和冷胁迫相关的代谢特征。这项工作为作物野生近缘种作为遗传资源的价值提供了概念验证，以识别适合提高作物抗逆性的遗传因素。
