Zhang Hexuan, Li Lingjun, Sun Xiaoqiang, Hou Benxiang, Luo Chunxiong
Center for Microscope Enhanced Dentistry, School of Stomatology, Capital Medical University, Beijing, China.
Department of Endodontics and Operative Dentistry, School of Stomatology, Capital Medical University, Beijing, China.
Biomed Microdevices. 2024 Jul 18;26(3):33. doi: 10.1007/s10544-024-00715-0.
Stem cells are crucial in tissue engineering, and their microenvironment greatly influences their behavior. Among the various dental stem cell types, stem cells from the apical papilla (SCAPs) have shown great potential for regenerating the pulp-dentin complex. Microenvironmental cues that affect SCAPs include physical and biochemical factors. To research optimal pulp-dentin complex regeneration, researchers have developed several models of controlled biomimetic microenvironments, ranging from in vivo animal models to in vitro models, including two-dimensional cultures and three-dimensional devices. Among these models, the most powerful tool is a microfluidic microdevice, a tooth-on-a-chip with high spatial resolution of microstructures and precise microenvironment control. In this review, we start with the SCAP microenvironment in the regeneration of pulp-dentin complexes and discuss research models and studies related to the biological process.
干细胞在组织工程中至关重要，其微环境极大地影响着它们的行为。在各种牙源性干细胞类型中，根尖乳头干细胞（SCAPs）在牙髓-牙本质复合体再生方面显示出巨大潜力。影响SCAPs的微环境线索包括物理和生化因素。为了研究最佳的牙髓-牙本质复合体再生，研究人员开发了几种可控的仿生微环境模型，从体内动物模型到体外模型，包括二维培养和三维装置。在这些模型中，最强大的工具是微流控微器件，即一种具有高空间分辨率微结构和精确微环境控制的芯片牙。在这篇综述中，我们从牙髓-牙本质复合体再生中的SCAP微环境入手，讨论与该生物学过程相关的研究模型和研究。
