Yamada Yoshiyuki, Terada Yuko, Yamanaka Rie, Enoyoshi Manami, Ito Keisuke
Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
Department of Food and Nutritional Sciences, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan; School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan.
Biochem Biophys Res Commun. 2024 Oct 30;731:150402. doi: 10.1016/j.bbrc.2024.150402. Epub 2024 Jul 14.
Given that the corneal epithelium is situated on the outermost part of the eye, its functions can be influenced by external temperatures and chemical substances. This study aimed to elucidate the expression profile of chemosensory receptors in corneal epithelial cells and analyze their role in eye function regulation. A comprehensive analysis of 425 chemosensory receptors in human corneal epithelial cells-transformed (HCE-T) revealed the functional expression of TRPV4. The activation of TRPV4 in HCE-T cells significantly increased the expression of membrane-associated mucins MUC1, MUC4, and MUC16, which are crucial for stabilizing tear films, with efficacy comparable to the active components of dry eye medications. The present study suggests that TRPV4, which is activated by body temperature, regulates mucin expression and proposes it as a novel target for dry eye treatment.
鉴于角膜上皮位于眼睛的最外层，其功能会受到外部温度和化学物质的影响。本研究旨在阐明角膜上皮细胞中化学感应受体的表达谱，并分析它们在眼功能调节中的作用。对人角膜上皮细胞转化株（HCE-T）中的425种化学感应受体进行的全面分析揭示了瞬时受体电位香草酸亚型4（TRPV4）的功能性表达。HCE-T细胞中TRPV4的激活显著增加了膜相关黏蛋白MUC1、MUC4和MUC16的表达，这些黏蛋白对稳定泪膜至关重要，其功效与干眼症药物的活性成分相当。本研究表明，由体温激活的TRPV4调节黏蛋白表达，并提出将其作为干眼症治疗的新靶点。
