Department of Regenerative Medicine Research, Foundation for Biomedical Research and Innovation, Kobe, Japan.
RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.
Stem Cells Dev. 2024 Sep;33(17-18):505-515. doi: 10.1089/scd.2024.0043. Epub 2024 Aug 9.
Intravascularly transplanted bone marrow cells, including bone marrow mononuclear cells (BM-MNC) and mesenchymal stem cells, transfer water-soluble molecules to cerebral endothelial cells via gap junctions. After transplantation of BM-MNC, this fosters hippocampal neurogenesis and enhancement of neuronal function. Herein, we report the impact of transplanted BM-MNC on neural stem cells (NSC) in the brain. Surprisingly, direct transfer of water-soluble molecules from transplanted BM-MNC and peripheral mononuclear cells to NSC in the hippocampus was observed already 10 min after cell transplantation, and transfer from BM-MNC to GFAP-positive cortical astrocytes was also observed. In vitro investigations revealed that BM-MNC abolish the expression of hypoxia-inducible factor-1α in astrocytes. We suggest that the transient and direct transfer of water-soluble molecules between cells in circulation and NSC in the brain may be one of the biological mechanisms underlying the repair of brain function.
经血管移植的骨髓细胞，包括骨髓单个核细胞（BM-MNC）和间充质干细胞，通过缝隙连接将水溶性分子转移到脑内皮细胞。BM-MNC 移植后，促进海马神经发生和神经元功能增强。在此，我们报告了移植的 BM-MNC 对脑内神经干细胞（NSC）的影响。令人惊讶的是，在细胞移植后 10 分钟，即可观察到水溶性分子从移植的 BM-MNC 和外周单个核细胞直接转移到海马的 NSC，而且也观察到从 BM-MNC 向 GFAP 阳性皮质星形胶质细胞转移。体外研究表明，BM-MNC 可消除星形胶质细胞中缺氧诱导因子-1α的表达。我们认为，循环中细胞与脑内 NSC 之间水溶性分子的短暂和直接转移可能是脑功能修复的生物学机制之一。
