巴基斯坦奎达一家三级护理医院的皮肤和软组织感染的细菌谱和抗生素敏感性。

The bacterial profile and antibiotic susceptibility in skin and soft tissue infections at a tertiary care hospital of Quetta, Pakistan.

机构信息

Centre for Advanced studies in Vaccinology and Biotechnology University of Balochistan, Quetta, Pakistan.

出版信息

J Pak Med Assoc. 2024 Jul;74(7):1249-1253. doi: 10.47391/JPMA.10261.

DOI:10.47391/JPMA.10261

PMID:39028049

Abstract

OBJECTIVES

To determine the bacterial profile and antibiotic susceptibility in skin and soft tissue infections among patients in a tertiary care setting.

METHODS

The cross-sectional cohort study was conducted at the Centre for Advanced Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta, Pakistan, from June 2021 to May 2022, and comprised bacteriainfected skin samples that were collected from the Bolan Medical Complex Hospital, Quetta, and the Sandeman Provincial Hospital, Quetta. The swab samples were immediately cultured, and positive samples were evaluated for biochemical tests, antibiotic susceptibility test and polymerase chain reaction. Data was analysed using SPSS 22.

RESULTS

Of the 800 samples, 598(74.7%) tested positive for pathogenic bacteria. Staphylococcus aureus accounted for 316(39.5%) infections, followed by clostridium perfringens 18.96(2.37%), escherichia coli 120(15.12%), pseudomonas aeruginosa 98(12.25%) and klebsiella pneumoniae 44(5.5%). Among all the infected samples, 380(47.5%) belonged to males, 218(27.25%) to patients aged 5-20 years, 448(56%) to the uneducated subjects, and 462(57.87%) to patients having lower socioeconomic status. Pseudomonas aeruginosa showed the highest level of resistance against all antibiotics.

CONCLUSION

Regular surveillance and proper use of antibiotics should be encouraged in hospitals to limit the spread of antibiotic resistance against pathogenic bacteria.

摘要

目的

确定三级医疗机构皮肤和软组织感染患者的细菌谱和抗生素敏感性。

方法

本横断面队列研究于 2021 年 6 月至 2022 年 5 月在巴基斯坦俾路支省大学疫苗学和生物技术高级研究中心进行，纳入了从俾路支省的博兰医疗综合体医院和桑德曼省级医院采集的感染细菌的皮肤样本。立即对拭子样本进行培养，对阳性样本进行生化试验、抗生素敏感性试验和聚合酶链反应评估。使用 SPSS 22 进行数据分析。

结果

在 800 个样本中，598 个（74.7%）检测出病原菌阳性。金黄色葡萄球菌感染占 316 例（39.5%），其次是梭状芽孢杆菌 18.96 例（2.37%）、大肠杆菌 120 例（15.12%）、铜绿假单胞菌 98 例（12.25%）和肺炎克雷伯菌 44 例（5.5%）。在所有感染样本中，380 例（47.5%）为男性，218 例（27.25%）为 5-20 岁患者，448 例（56%）为未受教育者，462 例（57.87%）为社会经济地位较低的患者。铜绿假单胞菌对所有抗生素的耐药性最高。