Department of Pathology, Bahria University Medical and Dental College, Karachi, Pakistan.
Department of Pathology, PNS Shifa, Karachi, Pakistan.
J Pak Med Assoc. 2024 Jul;74(7):1274-1279. doi: 10.47391/JPMA.10567.
To determine the immunohistochemical expression of Programmed cell Death Ligand 1 and intratumoural cluster of differentiation-8-positive T lymphocyte count in primary breast cancer cases, and to ascertain their association with different clinicopathological parameters.
The cross-sectional study was conducted at the Pakistan Navy Station Shifa Hospital, Karachi, from January 2020 to December 2021, and comprised patients of breast cancer regardless of age. Representative tissue blocks, both prospective and from the 2019 institutional archives, were exposed to immunohistochemical staining with Programmed cell Death Ligand 1 and intratumoural cluster of differentiation-8-positive T lymphocyte antibodies. Pathological and clinical records were used for assessing clinicopathological parameters. Data was analysed using SPSS 23.
Of the 70 women with mean age 52.04±12.54 years, 30(42.9%) expressed high Programmed cell Death Ligand 1 positivity, and 55(78.6%) revealed low intratumoural cluster of differentiation-8-positive T lymphocyte count, while 23 (32.9%), had both Programmed cell Death Ligand 1 high positivity and low intratumoural cluster of differentiation-8-positive T lymphocyte count. The association between Programmed cell Death Ligand 1 and intratumoural cluster of differentiation- 8-positive T lymphocytes was not significant (p=0.813). A strong significant association was observed between Programmed cell Death Ligand 1 expression and progesterone receptor negative status (p=0.008). No significant association was observed with any other clinicopathological parameter.
Programmed cell Death Ligand 1 high positivity and low intratumoural cluster of differentiation-8-positive T lymphocyte count were together observed in one-third of the breast cancer cases. A strong significant association existed between Programmed cell Death Ligand 1 high positivity and progesterone receptor negative status.
确定原发性乳腺癌病例中程序性细胞死亡配体 1 的免疫组织化学表达和肿瘤内分化群-8 阳性 T 淋巴细胞计数，并确定它们与不同临床病理参数的关系。
本横断面研究于 2020 年 1 月至 2021 年 12 月在卡拉奇巴基斯坦海军 Shifa 医院进行，研究对象为不论年龄的乳腺癌患者。采用免疫组织化学染色法对程序性细胞死亡配体 1 和肿瘤内分化群-8 阳性 T 淋巴细胞抗体对前瞻性和 2019 年机构档案中的代表性组织块进行染色。使用病理和临床记录评估临床病理参数。使用 SPSS 23 对数据进行分析。
在 70 名平均年龄为 52.04±12.54 岁的女性中，30 名（42.9%）表达高程序性细胞死亡配体 1 阳性，55 名（78.6%）显示低肿瘤内分化群-8 阳性 T 淋巴细胞计数，而 23 名（32.9%）既具有高程序性细胞死亡配体 1 阳性又具有低肿瘤内分化群-8 阳性 T 淋巴细胞计数。程序性细胞死亡配体 1 与肿瘤内分化群-8 阳性 T 淋巴细胞之间的关联不显著（p=0.813）。观察到程序性细胞死亡配体 1 表达与孕激素受体阴性状态之间存在强显著关联（p=0.008）。与任何其他临床病理参数均无显著关联。
三分之一的乳腺癌病例同时观察到程序性细胞死亡配体 1 高阳性和肿瘤内分化群-8 阳性 T 淋巴细胞计数低。程序性细胞死亡配体 1 高阳性与孕激素受体阴性状态之间存在强显著关联。
