Houston F, Sarhed G, Nyman S, Lindhe J, Karring T
J Clin Periodontol. 1985 Oct;12(9):716-27. doi: 10.1111/j.1600-051x.1985.tb01397.x.
The aim of the present investigation was to evaluate the regenerative potential of the periodontal tissues following tooth reimplantation using a model which excluded the dentogingival epithelium from the process of healing. Maxillary and mandibular incisors, premolars and molars of 5 monkeys were used. Following root filling of all experimental teeth, the teeth were divided into 3 experimental groups. In 1 group, the teeth were extracted following the elevation of full thickness flaps. The crowns were separated from the roots at the level of the buccal cemento-enamel junction and the roots immediately reimplanted into their sockets. The flaps were replaced and sutured to accomplish complete coverage of the roots. In a 2nd group, the teeth were subjected to the same experimental procedure, but in addition, the buccal alveolar bone was removed to about half its original height prior to root reimplantation. The teeth of the 3rd group were subjected to identical experimental procedures as for group II with the addition that the buccal root surfaces were planed to the level of the surgically created bone crest. The animals were sacrificed after 6 months of healing. The jaws were removed and histological specimens prepared for microscopic examination. The results showed that a complete fibrous re-attachment formed onto roots on which the original periodontal ligament tissue was preserved. This occurred irrespective of whether the roots were reimplanted into sockets with normal (group I) or reduced (group II) bone height. When the original periodontal ligament tissue was removed by root planing before reimplantation (group III), healing resulted in a significant amount of new connective tissue attachment. However, coronal to the newly formed fibrous attachment, the root surface frequently showed signs of resorption and particularly so in those roots which remained covered by the soft tissue during the entire course of healing. In the majority of the roots which perforated the covering soft tissue during the early phase of healing, the dentogingival epithelium had migrated apically into contact with the coronally generated fibrous attachment. In these cases, root resorption was never discernible. New bone formation occurred to a variable extent in the roots of groups II-III. No relationship was found, however, between the amount of connective tissue reattachment or new attachment and newly formed alveolar bone, which in turn indicates that bone tissue regrowth and periodontal ligament regeneration are unrelated phenomena.
本研究的目的是使用一种在愈合过程中排除牙龈上皮的模型，评估牙齿再植后牙周组织的再生潜力。使用了5只猴子的上颌和下颌切牙、前磨牙和磨牙。对所有实验牙齿进行根管充填后，将牙齿分为3个实验组。在1组中，在掀起全厚瓣后拔除牙齿。在颊侧牙骨质-釉质界水平将牙冠与牙根分离，牙根立即重新植入牙槽窝。将瓣复位并缝合以完全覆盖牙根。在第2组中，牙齿接受相同的实验程序，但此外，在牙根再植前将颊侧牙槽骨去除至其原始高度的约一半。第3组的牙齿接受与第2组相同的实验程序，另外将颊侧牙根表面刮至手术形成的牙槽嵴水平。愈合6个月后处死动物。取出颌骨并制备组织学标本用于显微镜检查。结果表明，在保留原始牙周膜组织的牙根上形成了完全的纤维性再附着。无论牙根是重新植入牙槽骨高度正常（第1组）还是降低（第2组）的牙槽窝中，都会出现这种情况。当在再植前通过牙根刮治去除原始牙周膜组织时（第3组），愈合导致大量新的结缔组织附着。然而，在新形成的纤维性附着的冠方，牙根表面经常出现吸收迹象，尤其是在整个愈合过程中仍被软组织覆盖的那些牙根中。在愈合早期穿透覆盖软组织的大多数牙根中，牙龈上皮已向根尖迁移并与冠方产生的纤维性附着接触。在这些情况下，从未观察到牙根吸收。第2 - 3组牙根中出现了不同程度的新骨形成。然而，在结缔组织再附着或新附着的量与新形成的牙槽骨之间未发现相关性，这反过来表明骨组织再生和牙周膜再生是不相关的现象。
