白色念珠菌形态突变体的遗传分析。

Genetic analysis of Candida albicans morphological mutants.

作者信息

Pomés R, Gil C, Nombela C

出版信息

J Gen Microbiol. 1985 Aug;131(8):2107-13. doi: 10.1099/00221287-131-8-2107.

Abstract

In contrast to some other strains, Candida albicans 1001 gave rise, upon UV irradiation, to mutants displaying a 'rough colony' morphology associated with a permanent alteration in morphogenesis which determined growth of the cells mostly as pseudohyphae. One of these mutants, C. albicans 1001FR, could form sectored (rough/smooth) colonies spontaneously, and with increasing frequency by treatment with mild UV doses (32-64 microJ mm-2). Rough sectors corresponded to stable 'rough-filamentous' strains which never segregated smooth strains. On the other hand, smooth sectors consisted mainly of yeast cells which could occasionally revert to a rough-filamentous phenotype. We suggest that C. albicans 1001 is heterozygous for some gene involved in the control of morphogenesis, and that the described mutants should be of help in the characterization of the genetic control of dimorphism in C. albicans.

摘要

与其他一些菌株不同，白色念珠菌1001经紫外线照射后产生了表现出“粗糙菌落”形态的突变体，这种形态与形态发生的永久性改变相关，这种改变决定了细胞主要以假菌丝形式生长。其中一个突变体，白色念珠菌1001FR，能够自发形成扇形（粗糙/光滑）菌落，并且通过用低剂量紫外线（32 - 64微焦耳/平方毫米）处理，形成频率会增加。粗糙扇形区域对应于稳定的“粗糙丝状”菌株，这些菌株从不分离出光滑菌株。另一方面，光滑扇形区域主要由酵母细胞组成，这些酵母细胞偶尔会回复到粗糙丝状表型。我们认为白色念珠菌1001在参与形态发生控制的某些基因上是杂合的，并且所描述的突变体应该有助于表征白色念珠菌中双态性的遗传控制。