Institute of Biotechnology & Genetic Engineering (Health Division), The University of Agriculture Peshawar, Peshawar, PO Box 25130, Pakistan.
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia.
Mol Biol Rep. 2024 Jul 20;51(1):827. doi: 10.1007/s11033-024-09765-2.
microRNAs (miRNAs) are small, noncoding RNA molecules, functioning either as oncogenes or tumor suppressor genes. SNPs in miRNAs might modify the expression of genes associated with miRNAs, enhancing susceptibility to breast cancer. Both miRNA-146a (rs2910164) and miRNA-196a (rs11614913) are identified and significantly associated with breast cancer risk in several ethnicities, but remains unexplored in Khyber Pakhtunkhwa population of Pakistan.
This study was aimed to check the relation of selected SNPs with breast cancer risk. The research cohort included 100 breast cancer patients and 100 healthy controls. All the participants were subjected for DNA extraction followed by T-ARMS PCR and gel electrophoresis.
The results revealed a strong association between risk allele (G) of miRNA-146a and increased risk of breast cancer (OR = 2.04, P = 0.0006). Similarly, heterozygous and mutant genotypes also indicated high risk and significant association with breast cancer risk (CG; OR = 0.51, 9 P = 0.0001) (GG; OR = 3.76, P = 0.04). However, risk allele (T) of miRNA-196a (rs11614913) failed to exhibit significant association with breast cancer risk (OR = 0.92 P = 0.68). Similarly, the heterozygous and mutant genotype did not show significant association with breast cancer risk (CT; OR = 0.52, P = 0.125 (TT; OR = 0.88, P = 0.84). Furthermore, miRNA-146a (rs2910164) and miRNA-196a (rs11614913) polymorphisms exhibited non-significant associations with family history (P = 0.34, P = 0.77), PR status (P = 0.310, P = 0.397), ER status (P = 0.992, P = 0.981), nodal status (P = 0.86, P = 0.90), and menstrual status (P = 0.97, P = 0.09). Notably, miRNA-196a showed a significant association with the metastasis group (P = 0.010) and cancer stages (P = 0.047).
In conclusion, this study highlights the association of miRNA-146a (rs2910164) polymorphism with breast cancer risk but suggested non-significant association of miRNA-196a (rs11614913) with breast cancer risk. However, these findings need to be confirmed through larger data set for more accurate result.
microRNAs (miRNAs) 是小的非编码 RNA 分子，作为癌基因或肿瘤抑制基因发挥作用。miRNAs 中的 SNP 可能会改变与 miRNAs 相关的基因的表达，从而增加患乳腺癌的易感性。miRNA-146a (rs2910164) 和 miRNA-196a (rs11614913) 都在多个种族中被确定并与乳腺癌风险显著相关，但在巴基斯坦开伯尔-普赫图赫瓦省的人群中尚未得到研究。
本研究旨在检查选定的 SNP 与乳腺癌风险的关系。研究队列包括 100 名乳腺癌患者和 100 名健康对照者。所有参与者均进行 DNA 提取，然后进行 T-ARMS PCR 和凝胶电泳。
结果显示，miRNA-146a 的风险等位基因 (G) 与乳腺癌风险增加之间存在很强的关联 (OR=2.04, P=0.0006)。同样，杂合和突变基因型也与乳腺癌风险呈高风险和显著相关 (CG; OR=0.51, 9 P=0.0001) (GG; OR=3.76, P=0.04)。然而，miRNA-196a (rs11614913) 的风险等位基因 (T) 未能显示与乳腺癌风险显著相关 (OR=0.92 P=0.68)。同样，杂合和突变基因型与乳腺癌风险也没有显著相关性 (CT; OR=0.52, P=0.125 (TT; OR=0.88, P=0.84)。此外，miRNA-146a (rs2910164) 和 miRNA-196a (rs11614913) 多态性与家族史 (P=0.34, P=0.77)、PR 状态 (P=0.310, P=0.397)、ER 状态 (P=0.992, P=0.981)、淋巴结状态 (P=0.86, P=0.90) 和月经状态 (P=0.97, P=0.09) 无显著关联。值得注意的是，miRNA-196a 与转移组 (P=0.010) 和癌症分期 (P=0.047) 显著相关。
总之，本研究强调了 miRNA-146a (rs2910164) 多态性与乳腺癌风险的关联，但 miRNA-196a (rs11614913) 与乳腺癌风险的关联无统计学意义。然而，这些发现需要通过更大的数据集进行验证，以获得更准确的结果。
