Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Key Laboratory for Medicinal Plant Chemistry and Chemical Biology, College of JunCao Science and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Key Laboratory for Medicinal Plant Chemistry and Chemical Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Ecotoxicol Environ Saf. 2024 Sep 1;282:116745. doi: 10.1016/j.ecoenv.2024.116745. Epub 2024 Jul 19.
Nitrogen (N), phosphorus (P) and potassium (K) are three macroelements in agriculture production, but their combined effects on arsenic (As) toxicity and its translocation in rice plants are not clear. In this study, an orthogonal rotation combination based on different N, P and K (NPK) concentration was first designed to examine their combined effect on the As toxicity, its transformation and migration in rice plants based on the hydroponic culture and pot soil culture. The results showed that 2.0 mg/L arsenite (As(III)) had obvious toxicity on the growth of indica LuYouMingZhan (LYMZ) and the optimal NPK concentration was 28.41, 6 and 50 mg/L based on the quadratic regression of the recovery rate of chlorophyll SPAD value of indica LYMZ. The optimal NPK combination significantly alleviated the physiological toxicity of As(III) on indica LYMZ rice seedling and decreased the accumulation of inorganic As in their roots and shoots by 23.8±1.8 % and 33.4±2.4 % respectively; further pot culture from different As(III) polluted soil showed that the optimal NPK combination significantly increased the dry weight of roots, stems, sheaths and leaves of indica LYMZ rice plants as well as yield indicators by 6.4 %-61.7 % and 7.1 %-89.8 % respectively, decreased the accumulation of As(III) and arsenate by 6.25 %-100 % and 12.36 %-100 % respectively in their roots, stems, sheaths, leaves, brans and kernels except As(III) concentration in their sheaths, decreased the accumulation of dimethylarsenate in their sheaths, leaves, brans and kernels, and had the best repair effect on the translocation of As species in 50 mg/kg As(III)-added soil. Our study provided a desirable strategy for alleviating As toxicity in paddy soil and reducing As pollution in rice plants.
氮（N）、磷（P）和钾（K）是农业生产中的三种大量元素，但它们对砷（As）毒性及其在水稻植株中迁移的综合影响尚不清楚。本研究采用基于不同 N、P 和 K（NPK）浓度的正交旋转组合，首次通过水培和盆栽土壤培养研究了其对砷毒性、转化和迁移的综合影响。结果表明，2.0mg/L 亚砷酸盐（As(III)）对籼稻绿油明占（LYMZ）的生长有明显毒性，基于籼稻 LYMZ 叶绿素 SPAD 值恢复率的二次回归，最佳 NPK 浓度为 28.41、6 和 50mg/L。最佳 NPK 组合显著缓解了 As(III)对籼稻 LYMZ 幼苗的生理毒性，使根和地上部无机 As 的积累分别降低了 23.8±1.8%和 33.4±2.4%；进一步从不同 As(III)污染土壤进行盆栽试验表明，最佳 NPK 组合显著增加了籼稻 LYMZ 根、茎、叶鞘和叶片的干重以及产量指标，分别增加了 6.4%-61.7%和 7.1%-89.8%，降低了根、茎、叶鞘、叶片、糠和糙米中 As(III)和砷酸盐的积累，分别降低了 6.25%-100%和 12.36%-100%，除了叶鞘中的 As(III)浓度外，还降低了叶鞘、叶片、糠和糙米中二甲砷酸盐的积累，对 50mg/kg As(III)添加土壤中 As 形态的迁移具有最佳的修复效果。本研究为缓解水稻土砷毒性和降低水稻植株砷污染提供了一种理想的策略。
