J Am Vet Med Assoc. 2024 Jul 18;262(S2):S7-S14. doi: 10.2460/javma.24.05.0333. Print 2024 Dec 1.
In vivo confocal microscopy (IVCM) is a unique imaging technique that permits noninvasive evaluation of the ocular surface on the cellular level. High-resolution images of all layers of the cornea are obtained in real-time with IVCM, and the acquired images are often comparable to ex vivo histochemical analysis of corneal biopsy specimens. The basic morphological features of the healthy living cornea as viewed by IVCM are reported in many domestic animal species, and the number of published descriptions of ocular surface pathologies in companion animals is progressively expanding. There is great potential for IVCM to improve the detection, characterization, and management of diverse ocular surface diseases in companion animals. This review summarizes several established and emerging clinical applications of IVCM in companion animal ocular surface disease, including infectious keratitis, corneal foreign bodies, corneal dystrophies and degenerations, ocular surface masses, corneal endotheliitis, pigmentary keratitis, and evaluation of corneal nerves.
体内共聚焦显微镜（IVCM）是一种独特的成像技术，可在细胞水平上对眼表进行非侵入性评估。IVCM 可实时获得角膜各层的高分辨率图像，所获得的图像通常可与角膜活检标本的离体组织化学分析相媲美。在许多家畜物种中，IVCM 观察到了健康活体角膜的基本形态特征，并且有关伴侣动物眼表疾病的描述数量也在逐渐增加。IVCM 有望提高伴侣动物各种眼表疾病的检测、特征描述和治疗水平。本文总结了 IVCM 在伴侣动物眼表疾病中的几种已建立和新兴的临床应用，包括感染性角膜炎、角膜异物、角膜营养不良和变性、眼表肿块、角膜内皮炎、色素性角膜炎和角膜神经评估。
