National Engineering Research Center for Chinese CRW (branch center), School of Life and Environmental Sciences, Shaoxing University, 900 Chengnan Road, Shaoxing, 312000, China.
Shaoxing Testing Institute of Food and Drug, National Center for Quality Inspection and Testing of Chinese Rice Wine, Shaoxing, 312000, China.
Environ Pollut. 2024 Oct 15;359:124577. doi: 10.1016/j.envpol.2024.124577. Epub 2024 Jul 19.
This study examines the impact of textile dye contamination on the structure of soil fungal communities near a Shaoxing textile dye factory. We quantified the concentrations of various textile dyes, including anthraquinone azodye and phthalocyanine, which ranged from 20.20 to 140.62 mg kg^-1, 102.01-698.12 mg kg^-1, and 7.78-42.65 mg kg^-1, respectively, within a 1000 m radius of the factory. Our findings indicate that as dye concentration increases, the biodiversity of soil fungi, as measured by the Chao1 index, decreases significantly, highlighting the profound influence of dye contamination on fungal community structure. Additionally, microbial correlation network analysis revealed a reduction in fungal interactions correlating with increased dye concentrations. We also observed that textile dyes suppressed carbon and nitrogen metabolism in fungi while elevating the transcription levels of antioxidant-related genes. Enzymes such as lignin peroxidase (LiP), manganese peroxidase (MnP), laccase (Lac), dye-decolorizing peroxidases (DyPs), and versatile peroxidase (VP) were upregulated in contaminated soils, underscoring the critical role of fungi in dye degradation. These insights contribute to the foundational knowledge required for developing in situ bioremediation technologies for contaminated farmlands.
本研究考察了绍兴某纺织染料厂附近土壤真菌群落结构受纺织染料污染的影响。我们定量分析了各种纺织染料的浓度，包括蒽醌偶氮染料和酞菁染料，其范围分别为 20.20-140.62mg/kg、102.01-698.12mg/kg 和 7.78-42.65mg/kg，在工厂半径 1000 米范围内。我们的研究结果表明，随着染料浓度的增加，土壤真菌生物多样性（用 Chao1 指数衡量）显著降低，这突出表明染料污染对真菌群落结构有深远影响。此外，微生物相关网络分析显示，随着染料浓度的增加，与真菌相互作用减少。我们还观察到，纺织染料抑制了真菌的碳氮代谢，同时提高了抗氧化相关基因的转录水平。受污染土壤中木质素过氧化物酶（LiP）、锰过氧化物酶（MnP）、漆酶（Lac）、染料脱色过氧化物酶（DyPs）和多功能过氧化物酶（VP）等酶的表达上调，这突出了真菌在染料降解中的关键作用。这些研究结果为开发受污染农田的原位生物修复技术提供了必要的基础理论知识。
